天津科技大学学报2016，Vol.31Issue(2)：20-25,6.DOI:10.13364/j.issn.1672-6510.20150054
提高根癌农杆菌介导黑曲霉转化效率的研究
Improvement of Transformation Efficiency of Aspergillus niger Mediated by Agrobacterium tumefaciens
摘要
Abstract
To improve theAgrobacterium tumefaciens-mediated transformation(ATMT)efficiency ofAspergillus niger,we performed some single-factor tests for five factors:ratio of conidia to bacterium,transformation media,inducing dissolved oxygen,inducing time and inducing concentration of acetosyringone(AS).The optimum conditions to improve the trans-formation efficiency were as follows:A tumefaciens was cultivated in 400,μmol/L AS at 28,℃ and 100,r/min for 5,h in 100,mL conical flask;co-cultivation was conducted at a 1﹕100,ratio of conidia to bacterium on nitrocellulose membraneat 24,℃ for 48,h.Under the above optimized conditions,the transformation efficiency ofA.niger achieved 35,transformants/107,conidia. The transformation efficiency was increased by 78%, compared with thatbefore optimization,and all the se-lected hygromycin B resistant transformants were genetically stable after 15 subcultures on complete medium.关键词
黑曲霉/根癌农杆菌/潮霉素B抗性Key words
Aspergillus niger/Agrobacterium tumefaciens/hygromycin B resistance分类
生物科学引用本文复制引用
曹张磊,王德培,张岚..提高根癌农杆菌介导黑曲霉转化效率的研究[J].天津科技大学学报,2016,31(2):20-25,6.基金项目
国家自然科学基金资助项目(31471725) （31471725）
