天津科技大学学报2016，Vol.31Issue(2)：31-35,56,6.DOI:10.13364/j.issn.1672-6510.20150088
迷迭香提取物对高脂膳食雌性果蝇致氧化应激的调控作用及机制
The Antioxidant Effect of Rosemary Extract on High Fat Diet-induced Damage in Female Drosophila melanogasterand its Regulatory Mechanism
摘要
Abstract
To study the effect of rosemary extract(ROE)on anti-aging and antioxidation in high-fat dietaryDrosophila melanogaster,Drosophila melanogasters were divided into three groups:the normal group:the high fat diet group(HF diet)and the experimental groups fed with different doses of rosemary extract(0.2,0.5,1.5,mg/mL).The medium was re-placed every three days and the number of the dead Drosophila melanogasters was recorded immediately,so the mean life-span and maximum lifespan were calculated.The variation of superoxide dismutase(SOD),catalase(CAT)and malondialde-hyde(MDA),as well as the mRNA expression levels of their encoding genes were determined after 45,days by Real-time PCR.It revealed that the lifespan of Drosophila melanogasters treated with resomary extract was extended significantly compared with the normal group. 0.2,mg/mL ROE could increase the enzyme activity of Mn-SOD significantly(P<0.05), and 1.5,mg/mL ROE increased the enzyme activity of Cu-Zn-SOD,Mn-SOD and CAT significantly(P<0.05)accompanied by a decrease of MDA content(P<0.01);with 0.5,mg/mL ROE,the mRNA expression levels of Cu-Zn-SOD,Mn-SOD, CAT and Nrf2 were up-regulated(P<0.05);with 1.5,mg/mL ROE,the mRNA expression levels of Mn-SOD and Nrf2,were up-regulated very significantly(P<0.01).It is concluded that the effect of rosemary extract on extending lifespan is likely to be related with the up-regulation of the gene expression level of endogenous antioxidant enzymes.关键词
迷迭香提取物/高脂膳食/黑腹果蝇/抗氧化/酶活力/基因表达Key words
rosemary extract/high fat diet/Drosophila melanogaster/antioxidant/enzyme activity/gene expression分类
轻工纺织引用本文复制引用
王红,陈纯,王轶菲,程立芳,蔡佳蓉,王浩..迷迭香提取物对高脂膳食雌性果蝇致氧化应激的调控作用及机制[J].天津科技大学学报,2016,31(2):31-35,56,6.基金项目
国家自然科学基金青年科学基金资助项目(31201322) （31201322）
