土壤与作物2016，Vol.5Issue(2)：120-127,8.DOI:10.11689/j.issn.2095-2961.2016.02.009
吉林省粳稻种质萌发期耐碱性和耐盐性综合评价
Comprehensive Evaluation of Rice(Oryza sativa japonica) Germplasm for Alkaline/Saline Tolerance at Germination Stage from Jilin Province,China
摘要
Abstract
In order to provide excellent germplasm resources for direct seeding and breeding for saline and alkaline stress tolerance, 60 representative local japonica rice cultivars from Jilin province, China, were evaluated for their tolerance to saline and alkaline stresses at seed germination stage based on the membership degree.The saline and alkaline stresses were simulated by 120 mM NaCl ( pH=6.98) and 30 mM Na2 CO3 ( pH=11.05) solutions respectively, and rice seeds were germinated on filter papers soaked with different stress solutions in a petri-dish (9cm diameter) .Five indices of seed germination including germination potential , germination rate, plumule length, root length and root number were measured under stressed or unstressed control conditions .The results revealed that the correlation of resistance to saline and alkaline reached highly significant level ( r=0.728**) , though there are varietal differ-ences.Relative plumule length, relative root length had good correlations with alkaline tolerance ( rBL=0.757 **, r RL=0.812 **) and saline tolerance (r BL =0.862**, rRL=0.781**), representing a useful set of simple and fast monitoring parameters for assess-ing saline/alkaline tolerance levels for individual rice varieties.Based on the membership degree, the varieties were divided into 6 grades of saline or alkaline tolerance:highly tolerant ( HT) , moderately tolerant ( MT) , tolerant ( T) , susceptible ( S) , moderately susceptible (MS), highly susceptible (HS) .We identified varieties Changbai 21 (HT/HT), Tonghe 835 (HT/HT), Ji Yujing (HT/HT), Changbai1 0 (HT/MT), Jinian 6 (MT/HT), Jinian 10 (MT/MT), Jijing 507 (MT/MT), Jijing 509 (MT/MT), Jijing 806 (MT/MT) and Dongdao 4 (MT/MT) as tolerant to both the saline and alkaline; Tonghe 99 (HT/S) and Tonghe 837 ( MT/S) as tolerant to saline but susceptible to alkaline;Jijing 105 ( MT/S) and Ji Nongda 828 ( MT/S) as tolerant to alkaline but susceptible to saline;Jiudao 51 (HSH/S), Ji Nongda 888 (HS/HS) and Tonghe 834 (HS/MS) as susceptible to both the saline and alkaline stresses.Furthermore, compared with the mid-late maturing or late maturing rice varieties , the earlym-edium maturing or medium maturing rice varieties in Jilin province showed better saline -alkaline tolerant performance.This finding may provide important reference for selecting parent sources in the saline -alkaline tolerant rice breeding programs.关键词
粳稻/耐盐性/耐碱性/相对根长/相对芽长/抗逆隶属度Key words
japonica rice/saline tolerance/alkaline tolerance/relative bud length/relative root length/membership degree分类
农业科技引用本文复制引用
冯钟慧,刘晓龙,姜昌杰,梁正伟..吉林省粳稻种质萌发期耐碱性和耐盐性综合评价[J].土壤与作物,2016,5(2):120-127,8.基金项目
中国科学院先导科技专项（XDA08040101）；中国科学院西部行动计划（Y2B2011001）． （）
