西安交通大学学报（医学版）2016，Vol.37Issue(3)：388-392,5.DOI:10.7652/jdyxb201603018
着丝粒蛋白W在脑胶质瘤中的表达及其对侵袭的作用
Expression of CENP-W in gliomas and its effect on invasion of gliomas cells
摘要
Abstract
Objective To investigate the expression of CENP-W in gliomas and its relationship with clinicopathological parameters and prognosis and to explore the effects of centromere protein W (CENP-W)on the invasion of gliomas cells.Methods The expressions of CENP-W in high-grade glioma tissues,low-grade glioma tissues,and adjacent brain tissues were detected by real-time fluorescence quantitative PCR and Western blotting. The correlation of the expression of CENP-W with clinicopathological parameters and prognosis was analyzed statistically.Human gliomas U2 5 1 cells in vitro were transfected with small interfering RNA to downregulate the expression of CENP-W.The invasion and migration capabilities of gliomas cancer cells were assessed by Transwell assays.Results The expression level of CENP-W was significantly higher in glioma tissues than in normal tissues. There was a positive correlation between the three protein expression levels and the pathological grade of gliomas. CENP-W siRNA was successfully transfected into U2 5 1 cells.Compared with those of the cells transfected with the scramble siRNA and control cells,the invasive and migration activities were inhibited in the U2 5 1 cells transfected with CENP-W siRNA.The Kaplan-Meier analysis and Log-Rank test showed significant differences in progress free survival (PFS)between the CENP-W high-expression and low-expression groups.Conclusion The expression level of CENP-W was positively correlated with the pathological grade of gliomas and CENP-W can promote glioma cell invasion.It implicates that CENP-W can be a novel target in gliomas treatment.关键词
着丝粒蛋白 W/脑胶质瘤/侵袭Key words
centromere protein W (CENP-W)/glioma/invasion分类
医药卫生引用本文复制引用
吴淼经,汲乾坤,范阳华,吕世刚,叶敏华,吴雷,祝新根..着丝粒蛋白W在脑胶质瘤中的表达及其对侵袭的作用[J].西安交通大学学报（医学版）,2016,37(3):388-392,5.基金项目
江西省自然科学基金计划项目(No.20132BAB205043)、江西省教育厅科学技术研究项目(No.GJJ14066) Supported by the Natural Science Foundation of Jiangxi Province (No.20132BAB205043)and Jiangxi Province Department of Education Science and Technology Research Project (No.GJJ14066) （No.20132BAB205043）
