中国组织工程研究2016，Vol.20Issue(16)：2317-2323,7.DOI:10.3969/j.issn.2095-4344.2016.16.005
骨内置入硫酸钙产品的生物相容性与安全性评价
Biocompatibility and security of calcium sulfate bone substitutes
摘要
Abstract
BACKGROUND:It is a great potential study that calcium sulfate product loaded with antibiotics is developed, but this product is not systematicaly studied and its biocompatibility and security need to be further studied. <br> OBJECTIVE:To evaluate the biocompatibility and safety of vancomycin- or gentamicin-loaded calcium sulfate bone. <br> METHODS: (1) Hemolysis test: vancomycin-loaded, gentamicin-loaded calcium sulfate extracts, double distiled water and saline were added into rabbit anticoagulant blood samples. (2) Micronucleus test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts, cyclophosphamide and normal saline solution were intraperitonealy injected to mice, respectively. (3) Acute toxicity test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts, and normal saline solution were intraperitonealy injected to mice, respectively. (4) Pyrogen test: the mice were injected vancomycin-loaded and gentamicin-loaded calcium sulfate extractsvia the ear vein. (5) Intradermal stimulation test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts were respectively injected into the unilateral spine of rabbits, respectively. (6) Intramuscular implantation test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts were respectively injected to the dorsal muscle of rabbits. (7) Intraosseous implantation test: vancomycin-loaded and gentamicin-loaded calcium sulfate were implanted into the necrotic femoral bone of rabbits. <br> RESULTS AND CONCLUSION:Both vancomycin-loaded and gentamicin-loaded calcium sulfate products, which have no hemolytic reaction, genetic toxicity, acute toxicity, pyrogen reaction and skin irritation, are considered to have good biocompatibility and safety.关键词
生物材料/骨生物材料/载万古霉素硫酸钙/载庆大霉素硫酸钙/生物相容性/安全性/骨内植入物分类
医药卫生引用本文复制引用
邱奕雁,陈扬,周文钰,夏晓龙,杨欣建..骨内置入硫酸钙产品的生物相容性与安全性评价[J].中国组织工程研究,2016,20(16):2317-2323,7.基金项目
2014年建设中医药强省立项资助项目(20141257)中药牛淫合剂干预骨代谢与骨密度的研究.@@@@the Provincial-Level Funding Project for Strengthening the Development of Traditional Chinese Medicine in 2014, No.20141257 （20141257）
