中国组织工程研究2016，Vol.20Issue(16)：2324-2332,9.DOI:10.3969/j.issn.2095-4344.2016.16.006
外科生物补片修复退变椎间盘纤维环
Surgical biomaterials to repair the ruptured annulus fibrosus due to intervertebral disc degeneration
摘要
Abstract
BACKGROUND:In recent years, animal models of lumbar disc degeneration have been popularized to explore the effect of tissue engineering, cel engineering and genetic engineering technologies on intervertebral disc degeneration. <br> OBJECTIVE: To investigate the influence of biological patches on rabbit anulus fibrosus repair. <br> METHODS:L3-4, L4-5, L5-6 segments from rabbits were randomly divided into normal control group (the intervertebral disc was exposed correspondingly), control group (the annulus fibrosus was only cut with a surgical scalpel) and experimental group (the annulus fibrosus was cut and sutured with the surgical biomaterial). Six rabbits were selected randomly to take the lumbar X-ray and MRI preoperatively and 1, 2, 4, 8, 12 weeks postoperatively; one rabbit was chosen preoperatively and three rabbits selected respectively at 1, 2, 4, 8, 12 weeks postoperatively to execute hematoxylin-eosin and type II colagen immunohistochemistry. <br> RESULTS AND CONCLUSION:At postoperative 1, 2 and 4 weeks, the disc height index decreased significantly in the control and experimental groups. MRI and histopathological examination showed that the T2WI signal intensity and hematoxylin-eosin grading were both increased significantly in the control and experimental groups at 2 weeks after surgery (P < 0.05). With time, the number of nucleus pulposus cels gradualy reduced in the control and experimental groups. Annulus fibrosus defects were filed with granulation and fibrous tissues, and the biofilm was tightly fused with the annulus fibrosus. Findings from the type II colagen immunohistochemical staining showed that the histological staining of the nucleus pulposus was gradualy changed from positive to negative in the experimental and control groups. Therefore, cutting the annulus fibrosus can lead to severe disc degeneration at early period, and surgical biomaterials can be integrated with the annulus fibrosus wel to seal annulus fibrosus defects and further prevent nucleus pulposus protrusion. However, this approach cannot restrain the continuous process of disc degeneration.关键词
生物材料/骨生物材料/外科生物材料/椎间盘退变/椎间盘高度指数/磁共振T2加权像/免疫组织化学染色/新疆维吾尔自治区自然科学基金分类
医药卫生引用本文复制引用
朱旭,马原,孟祥玉,田慧中..外科生物补片修复退变椎间盘纤维环[J].中国组织工程研究,2016,20(16):2324-2332,9.基金项目
新疆维吾尔自治区自然科学基金资助项目(2014211C135).@@@@the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No.2014211C135 （2014211C135）
