中国组织工程研究2016，Vol.20Issue(16)：2405-2411,7.DOI:10.3969/j.issn.2095-4344.2016.16.017
3D生物打印构建聚乳酸羟基乙酸/纳米羟基磷灰石支架骨形态发生蛋白2缓释复合体的实验研究
3D-bioprinting manufacturing polylactic-co-glycolic acid/nano-hydroxyapatite scaffold/bone morphogenetic protein-2 sustained release composite
摘要
Abstract
BACKGROUND:Tissue-engineered bone scaffold fabricated by 3D-bioprinting technique has good controlability in morphology and structure. However, construction of tissue-engineered bone/cel growth factor complex and time-dose effect of sustained-release factors are needed to be further researched. <br> OBJECTIVE:To fabricate a sustained-release composite of polylactic-co-glycolic acid (PLGA)/nano-hydroxyapatite (n-HA) scaffold carrying bone morphogenetic protein-2 (BMP-2) using 3D-bioprinting technique, and test the biological properties of the PLGA/n-HA scaffold carrying BMP-2 and the sustained-release properties, thereby to discuss its feasibility as the tissue-engineered bone scaffold composite. <br> METHODS:Temperature-sensitive chitosan hydrogel was prepared using chitosan andβ-glycerophosphate to construct a sustained-release composite, chitosan nanoparticles carrying BMP-2 . 3D-bioprinting technique was utilized to fabricate the PLGA/n-HA scaffold carrying BMP-2. Biological features of the scaffold composite were tested, and time-dose effect of BMP-2 sustained-release was observed. <br> RESULTS AND CONCLUSION:The average pore size of the scaffold-cytokine composite was (431.31±18.40)μm, and the porosity was (73.64±1.82)%. The cumulative release rate of BMP-2 from the scaffold-cytokine composite that effectively controled the burst release during 48 hours and 30 days were suitable for the physiological needs. In conclusion, the porosity, pore size, release property, degradation rate, and mechanical strength of the scaffold-cytokine composite al meet the biological requirements of tissue-engineered bone construction.关键词
生物材料/缓释材料/聚乳酸羟基乙酸/纳米羟基磷灰石/3D生物打印技术/缓释载体/支架/壳聚糖/水凝胶/纳米球/山东省自然科学基金分类
医药卫生引用本文复制引用
臧晓龙,孙健,李亚莉,陈立强,杨学财,梁立卿,杜国庆..3D生物打印构建聚乳酸羟基乙酸/纳米羟基磷灰石支架骨形态发生蛋白2缓释复合体的实验研究[J].中国组织工程研究,2016,20(16):2405-2411,7.基金项目
山东省自然科学基金项目(ZR2012HM069)缩略语.@@@@the Natural Science Foundation of Shandong Province, No. ZR2012HM069 （ZR2012HM069）
