中国组织工程研究2016，Vol.20Issue(16)：2412-2417,6.DOI:10.3969/j.issn.2095-4344.2016.16.018
魔芋葡甘聚糖/纳米羟基磷灰石/胶原复合材料构建组织工程椎间盘纤维环支架
Tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan/nano-hydroxyapatite/collagen composite material
摘要
Abstract
BACKGROUND:Tissue-engineered transplantation technique has become an ideal therapeutic regimen for degenerative disc diseases through reconstituting the biological functions of the degenerated intervertebral discs. <br> OBJECTIVE:To construct a novel tissue-engineered annular fibrosus scaffold. <br> METHODS:Konjac glucomannan, nano-hydroxyapatite and colagen were used to fabricate a new tissue-engineered annular fibrosus scaffold by wet spinning, chemical crosslinking, and freeze drying methods. Afterwards, X-ray diffraction and Fourier transform infrared spectrometer were used to analyze the scaffold qualitative components, physico-chemical property, biomechanical performance and cytocompatibility. <br> RESULTS AND CONCLUSION:The bionic scaffold had a three-dimensional porous structure, with the average pore size of (425.8±47.3) μm, the average porosity of (73.4±5.6)%, and the water absorption of (718.6±24.3)%. In addition, the compressive strength of the scaffold was similar with that of the natural annular fibrosus. More importantly, the scaffold had good biocompatibility without cytotoxicity. These results show that the tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan, nano-hydroxyapatite and colagen has proper three-dimensional porous structure, biocompatibility, porosity, water absorption and biomechanical strength.关键词
生物材料/纳米材料/纳米羟基磷灰石/胶原/椎间盘/魔芋葡甘聚糖/纤维环分类
医药卫生引用本文复制引用
庄颖,陈希亮,李勇,陈庆华,潘兴华,徐永清..魔芋葡甘聚糖/纳米羟基磷灰石/胶原复合材料构建组织工程椎间盘纤维环支架[J].中国组织工程研究,2016,20(16):2412-2417,6.基金项目
成都军区医学科学技术研究计划项目(C14013).@@@@Chengdu Military Medical Science and Technology Research Project, No. C14013 （C14013）
