中国病理生理杂志2016，Vol.32Issue(4)：658-664,7.DOI:10.3969/j.issn.1000-4718.2016.04.013
沉默URG11基因对前列腺癌LNCaP细胞增殖、迁移与侵袭的影响
Effect of URG11 gene silencing on proliferation and invasion abilities of human prostate cancer cells
摘要
Abstract
AIM:To investigate the expression of up-regulated gene 11 ( URG11 ) in prostate cancer cell line and the effect of URG11 siNRA on the proliferation and invasion of human prostate cancer LNCaP cells.METHODS:The mRNA and protein levels of URG11 in prostate cancer cell lines and normal prostate epithelial cell line were evaluated by real-time PCR and Western blot.LNCaP cells were transfected with designed siRNA using the liposome method.The prolif-eration, apoptosis, migration and invasion abilities of the LNCaP cells were evaluated by MTS assay, flow cytometry, wound-healing assay and Transwell assay.RESULTS:The expression of URG11 at mRNA and protein levels in the DU145, PC3, LNCaP cell lines was significantly higher than that in RWPE-1 cell line.Compared with the control group, the proliferation of LNCaP cells with URG11 siRNA was stagnant in G1/S phase and induced apoptosis.The proliferation of LNCaP cells at 0 h, 24 h, 48 h and 72 h was inhibited after URG11 expression was down-regulated (P<0.05).Transwell assay showed that migration (P<0.05) and invasion (P<0.05) were also inhibited.CONCLUSION:URG11 is highly expressed in prostate cancer.Silencing of URG11 significantly inhibits the proliferation and invasion and induces apoptosis of LNCaP cells.关键词
上凋基因11/前列腺癌/RNA干扰/肿瘤侵袭Key words
Up-regulated gene 11/Prostate cancer/RNA interference/Neoplasm invasion分类
医药卫生引用本文复制引用
潘斌,邓志海,吴友科,梁蔚波,曾传蓉,刘海平..沉默URG11基因对前列腺癌LNCaP细胞增殖、迁移与侵袭的影响[J].中国病理生理杂志,2016,32(4):658-664,7.基金项目
广东省医学科学技术研究基金资助项目( No.A2015557) （ No.A2015557）
中央高校基本科研业务费专项资金资助项目( No. 21613317) （ No. 21613317）
