中国病理生理杂志2016，Vol.32Issue(4)：762-768,7.DOI:10.3969/j.issn.1000-4718.2016.04.030
GDF11在体外扩增CD8+记忆干性T细胞的作用
Effect of GDF11 on expansion of CD8 +memory stem T cells
摘要
Abstract
AIM:To investigate the effect of growth differentiation factor 11 ( GDF11 ) on the expansion of CD8 +memory stem T cells ( Tscm) and to further improve the effect of adoptive immunotherapy.METHODS:Healthy human peripheral blood mononuclear cells ( PBMCs) were isolated by density gradient centrifugation at first.Among the i-solated PBMCs, CD8 +T cells were further purified with MACS microbeads.The CD8 +T cells were then randomly divided into experimental groups and control group.The same volume of different concentrations of GDF11 were added into the ex-perimental groups, and the same volume of PBS solution was added into the control group.Finally, the expansion of Tscm in experimental groups and control group was measured by flow cytometry at several time points.RESULTS:GDF11 sig-nificantly increased the number of Tscm in CD8 +T cells in vitro expansion and also dramatically increased the ratio of Tscm in CD8 +T cells.Furthermore, 400 μg/L GDF11 treatment for 3 weeks was the optimal condition to induce CD8 +Tscm. CONCLUSION:GDF11 effectively increases the number and ratio of Tscm in the CD8 +T cells in cell culture growth, thereby creating a new strategy to further improve the efficiency of adoptive immunotherapy.关键词
生长分化因子11/CD8+记忆干性T细胞/过继免疫治疗Key words
Growth differentiation factor 11/CD8 +memory stem T cells/Adoptive immunotherapy分类
医药卫生引用本文复制引用
马星如,陈颖诗,林颖彤,张旭,罗海华,刘超,潘婷..GDF11在体外扩增CD8+记忆干性T细胞的作用[J].中国病理生理杂志,2016,32(4):762-768,7.基金项目
国家自然科学基金资助项目(No.31500740) （No.31500740）
广东省引进创新科研团队项目(No.2009010058) （No.2009010058）
"十二五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项基金资助项目(No.2013ZX10001004) （No.2013ZX10001004）
