中国肺癌杂志2016，Vol.19Issue(4)：169-176,8.DOI:10.3779/j.issn.1009-3419.2016.04.11
尼古丁诱导肺癌细胞上皮间质转化促进其侵袭转移
Nicotine Induced Lung Cancer Cells Epithelial-mesenchymal Transition and Promote Its Vitro Invasion Potential
摘要
Abstract
Background and objective Our previous study found that nicotine could induce lung cancer cell epithelial-mesenchymal transition (EMT). The aim of this study is to explore the relationship between nicotine-induced EMT and lung cancer invasion and metastasis. Methods Real-time PCR and Western blot were used to detect the expression changes of EMT-related markers, E-cadherin and Vimentin, in A549 lung cancer cells treated with nicotine;hTe transposition ofβ-catenin protein expression was determined by immunolfuorescence;Scratch test and Transwell invasion assay were used to detect the effects of nicotine on lung cancer cell migration and invasion. Results Nicotine can signiifcantly down-regulate the expressional level of E-cadherin mRNA and protein of A549 cells in a manner of dose and time-dependent (P<0.01, P<0.01);Nicotine can signiifcantly up-regulate the expressional level of Vimentin mRNA and protein of A549 cells in a manner of dose and time-dependent (P<0.01, P<0.01);Immunolfuorescence results showed thatβ-catenin protein was signiifcantly transfered to nucleus;Scratch test and Transwell assay showed that Nicotine could remarkably increase the migration and invasion poten-tial of lung cancer cells (P<0.01, P<0.01). Conclusion Nicotine can induce cancer cells EMT, and promote the invasion and metastasis ability of lung cancer cells.关键词
肺肿瘤/尼古丁/上皮间质转化/侵袭Key words
Lung neoplasms/Nicotine/Epithelial-mesenchymal transition/Invasion引用本文复制引用
侯艳须,沈旺,周清华,李雪冰,潘振华,祖玲玲,范亚光,尤嘉琮,王玉丽,王岷,陈沛锐..尼古丁诱导肺癌细胞上皮间质转化促进其侵袭转移[J].中国肺癌杂志,2016,19(4):169-176,8.基金项目
本研究受国家自然科学基金（No.81572288）和教育部高等学校博士点优先发展领域基金（No.20131202130001）资助 （）
