中国感染控制杂志2016，Vol.15Issue(4)：217-221,5.DOI:10.3969/j.issn.1671-9638.2016.04.001
临床分离非伤寒沙门菌耐药性及对头孢曲松耐药机制
Antimicrobial resistance and mechanisms of ceftriaxone resistance in clini-cally isolated nontyphoidal Salmonella
摘要
Abstract
Objective To investigate antimicrobial resistance and ceftriaxone resistance mechanisms in clinically isolated nontyphoidal Salmonella(NTS),and provide evidence for the prevention and control of NTS infection and rational use of antimicrobial agents.Methods 108 NTS isolates were isolated from stool specimens of outpatients with acute diarrhea in the Second Hospital of Tianjin Medical University and Tianjin Medical University General Hospital from May to October of 2014,NTS were performed antimicrobial susceptibility testing;non-ceftriaxone-susceptible isolates were typed by serological,multilocus sequence (MLST),and pulsed-field gel electrophoresis (PFGE)methods,extended-spectrumβ-lactamase (ESBL)detection and AmpC genes were detection.Results Among 108 NTS isolates,mono-drug resistance rate to 11 antimicrobial agents was 49.07% (n= 53),multidrug resistance rate was17.59% . Susceptibility rates to nalidixic acid,levofloxacin,ciprofloxacin,ceftriaxone,and ertapenem were 61.11% ,66.67% ,68.52% ,97.22% ,and 100.00% respectively. Three non-ceftriaxone-susceptible NTS isolates were detected,2 were ST11 Salmonellaenterica serotype (Sa8709,Sa8771),1 was ST34 Salmonellatyphimurium serotype(Sa8763). Cluster analysis of PFGE revealed that Sa8709 was highly similar to Sa8771 strains(91 .70% ), but the similarity to Sa8763 was low(55.80% );Sa8709 strain carried CTX-M gene,Sa8771 strain carried CTX-M and TEM genes,Sa8763 strain carried OXA gene. Conclusion Clinically isolated NTS in this area are low resistant to fluoroquinolones,multidrug resistant strains carrying ESBLs have emerged.关键词
非伤寒沙门菌/多位点序列分型/脉冲场凝胶电泳/超广谱β-内酰胺酶/氟喹诺酮/抗药性,微生物/耐药性Key words
nontyphoidal Salmonella/multilocus sequence typing/pulsed-field gel electrophoresis/extended-spectrumβ-lactamase/fluoroquinolone/drug resistance/microbial/drug resistance分类
医药卫生引用本文复制引用
师伟,夏琳林,刘晓霞,韩双羽,杨杰,赵展,王玉宝..临床分离非伤寒沙门菌耐药性及对头孢曲松耐药机制[J].中国感染控制杂志,2016,15(4):217-221,5.基金项目
天津市卫生局科研基金资助项目(2013KZ112) （2013KZ112）
