局解手术学杂志2016，Vol.25Issue(4)：235-239,5.DOI:10.11659/jjssx.10E015172
软骨终板干细胞动物体内成骨／软骨的初步研究
Study on osteogenic/chondrogenic differentiation potential of cartilage endplate derived stem cells in vivo
摘要
Abstract
Objective To observe the osteogenic and/or chondrogenic differentiation potential of cartilage endplate derived stem cells ( CESCs) .Methods The cartilage endplate ( CEP) was obtained from 5 patients who underwent posterior discectomy procedure for the lumber degenerative disease.The agarose culture was used to select CESCs.The expanded CESCs were injected into the hydroxyapatite ceram-ic(HA) and incubated for 7 days.After that,the HA contained CESCs were implanted into the subcutaneous of nude mouse for 7 weeks.Then the HA was obtained and sliced for the specific stain and immunohistochemisty.The expanded CESCs were injected into the degenerated in-tervertebral disk of the rabbit for 16 weeks.Then MRI was used to detect the repair of the degenerated disc.Results The metachromasia of toluidin blue indicated that cartilage matrix was formed in the vacant space of HA.Mallory stain indicated that there was bone matrix was formed.The results of immunohistochemistry indicated that there was more collageⅠand collageⅩ,and a small quantity of collage Ⅱ.MRI results indicated that the CESCs could be induced into the chondrocyte-like cells in the rabbit intervertebral disk.Conclusion The CESCs could be induced into the bone/cartilage in vivo.关键词
椎间盘/干细胞/软骨终板/诱导Key words
intervertebral disc/stem cells/cartilage endplate/induce分类
医药卫生引用本文复制引用
刘兰涛,朱瑜洁,黄博,李长青,周跃..软骨终板干细胞动物体内成骨／软骨的初步研究[J].局解手术学杂志,2016,25(4):235-239,5.基金项目
国家自然科学基金 （）
