局解手术学杂志2016，Vol.25Issue(4)：240-243,4.DOI:10.11659/jjssx.01E016024
hTNFα／CHO基因工程细胞的构建及其分泌蛋白功能的检定
Construction of hTNFα/CHO genetic engineered cell and verification of its protein secretion function
摘要
Abstract
Objective To construct the genetic engineered cell line which can continuously secrete human tumor necrosis factor ( hTNFα) in Chinese Hamster Ovary cells( CHO cells) ,and observe the change of its protein secretion function.Methods Constructed plas-mid which carries hTNFαgene expression through vector GV141.Selected stable transfection cell lines by G418 and transfection with lipo-fectamine.Identified its gene expression with Real-Time PCR,and identified its protein secretion by ELISA.Results GV141-hTNFαexpres-sion vectors were constructed successfully which were proved by sequence alignment.Real-Time PCR proved that it contained hTNFαgene in hTNFα/CHO cell line.ELIAS identification results showed that the cell lines can continuously secrete hTNFαwithin a certain cell propaga-tion.Conclusion The hTNFα/CHO cell line can continuously secrete human tumor necrosis factor within a certain cell propagation.关键词
基因工程细胞/人肿瘤坏死因子/转染/蛋白分泌Key words
genetic engineered cell/human tumor necrosis factor/transfection/protein secretion分类
医药卫生引用本文复制引用
田磊,潘静坤,施明,薛毅珑..hTNFα／CHO基因工程细胞的构建及其分泌蛋白功能的检定[J].局解手术学杂志,2016,25(4):240-243,4.基金项目
国家“863”计划 （）
