局解手术学杂志2016，Vol.25Issue(4)：244-247,4.DOI:10.11659/jjssx.01E016075
低浓度氧化铈纳米材料对肝癌细胞增殖能力的影响
Effect of low concentration of cerium oxide nanomaterials on hepatocellular carcinoma cell proliferation
摘要
Abstract
Objective To study the impact of different low concentrations of cerium oxide for hepatocellular carcinoma cell prolifera-tion.Methods Three different types of hepatoma cells (Huh7, HepG2,7721) were cultured,and added different concentrations of cerium oxide (0.005,0.01,0.05,0.1,1 μg/mL),of which the cell proliferation was detected by CCK8.The apoptosis-related genes was detected by qRT-PCR technology.The cell cycle was analyzed by flow cytometry.And the effect of low concentration cerium oxide on hepatocellular carci-noma cells tumorigenicity was confirmed by the nude mice experiments.Results CCK8 experiment showed that low concentrations of cerium oxide could promote proliferation of hepatocellular carcinoma cell, especially in concentration of 0.01μg/mL.The qRT-PCR showed that low concentration of cerium oxide could inhibit the apoptosis of hepatocellular carcinoma cell.The flow cytometry analysis had not found any effect of cerium oxide on cell cycle.The tumorigenicity experiments confirmed that low concentrations of cerium oxide could enhance the tumorigenic ability of hepatocellular carcinoma cell.Conclusion Low concentration of cerium oxide can significantly improve the proliferation of liver cancer cells.关键词
氧化铈纳米材料/肝癌细胞/增殖能力/流式细胞术Key words
cerium oxide/hepatocellular carcinoma cell/proliferation/flow cytometry analysis分类
医药卫生引用本文复制引用
程姮,廖忠莉,陈虹燕,王涛,郭红..低浓度氧化铈纳米材料对肝癌细胞增殖能力的影响[J].局解手术学杂志,2016,25(4):244-247,4.基金项目
国家自然科学基金 （）
