局解手术学杂志2016，Vol.25Issue(4)：248-250,251,4.DOI:10.11659/jjssx.12E015112
大鼠角膜缘干细胞分离培养及其横向分化能力初探
Isolation and culture of rat limbal stem cells and research on trans differation capacity
摘要
Abstract
Objective To isolate and culture the rat limbal stem cells ( LSCs) in vitro,and investigate briefly their capacity for transdif-ferentiation into neural stem cells ( NSCs) with cytokine EGF,bFGF and RA.Methods LSCs derived from rats were cultured and identified by immunohistochemistry in vitro.LSCs were induced to differentiate into NSCs in the presence of EGF (20 ng/mL) ,bFGF (10 ng/mL) and with or without of RA(group 1 or group 2)(25 ng/mL) for seven days.Cultures without factors were used as control group.Then the neural marker Nestin of the coultured cells were measured by immunohistology staining.Furthermore,the positive cell rate was counted under micro-scope between the 2 groups and analyzed by statistical software.Results It showed that P63 was positive in LSCs.Nestin in both of the dif-ferentiation groups was positive at the rate of (77.01 ±6.32)%and (84.01 ±5.43)%respectively,of which the second group was higher than the first one (P<0.05).However,it was negative in the control group.A band of Nestin protein from cells was detected by western blot assay.Conclusion LSCs are successfully isolated and cultured in vitro.LSCs could be induced to differentiate into NSCs in the presence of EGF and bFGF.Moreover,the differentiation capability is enhanced in the condition of RA.关键词
大鼠角膜缘干细胞/神经干细胞样细胞/表皮生长因子/碱性成纤维细胞生长因子/全反式维甲酸Key words
limbal stem cells/neural stem cell-like cells/epidermal growth factor/basic fibroblast growth factor/all-transretinoic acid分类
医药卫生引用本文复制引用
严会文,陈剑英,高杰,廖文萍,苏敏,胡蓉..大鼠角膜缘干细胞分离培养及其横向分化能力初探[J].局解手术学杂志,2016,25(4):248-250,251,4.基金项目
贵州省优秀科技教育人才省长专项资金项目（黔省专合字【2012】41号）；贵州省科技厅、贵阳医学院联合基金 （）
