中国康复理论与实践2016，Vol.22Issue(4)：417-421,5.DOI:10.3969/j.issn.1006-9771.2016.04.010
两种组织透明技术在免疫荧光染色观察脊髓3D结构中的应用
Application of Two Kinds of Tissue Clearing Method in Observing 3D Imaging of Spinal Cord with Immunofluo-rescent Staining
摘要
Abstract
Objective To compare the application of CUBIC and iDISCO clearing methods in observing 3D imaging of spinal cord with immunofluorescent staining. Methods 1 mm thick spinal cord coronal sections were processed with CUBIC and iDISCO, respectively. The neurofilament (NF) protein was detected by immunofluorescent staining and then was observed by a laser confocal microscope. Results Compared with CUBIC, iDISCO had the advantages of shorter time, higher transparency (F=6.64, P<0.01), and deeper penetration (F=5117.55, P<0.01). Conclusion Immunofluorescent staining combined with iDISCO could completely observe the spinal axons with shorter time and better stain effect.关键词
脊髓/透明/轴突再生/CUBIC/iDISCO/大鼠Key words
spinal cord/transparency/axon regeneration/CUBIC/iDISCO/rats分类
医药卫生引用本文复制引用
段红梅,尚俊奎,郝鹏,李千千,杨飞祥,杨朝阳,李晓光..两种组织透明技术在免疫荧光染色观察脊髓3D结构中的应用[J].中国康复理论与实践,2016,22(4):417-421,5.基金项目
1.国家“863”计划项目(No.2012AA020506)；2.国家自然科学基金面上项目(No.31271037)；3.国家自然科学基金国际合作与交流项目(No.31320103903)；4.“十二五”国家科技支撑计划项目(No.2012BAI17B04)；5.高等学校全国优秀博士学位论文作者专项资金资助项目(No.201356)；6.国家国际科技合作专项项目(No.2014DFA30640)；7.国家自然科学基金委员会资助项目(No.31130022)。 （No.2012AA020506）
