中国康复理论与实践2016，Vol.22Issue(4)：422-427,6.DOI:10.3969/j.issn.1006-9771.2016.04.011
丁苯酞对1-甲基-4-苯基吡啶离子诱导细胞自噬的影响
Effect of Butylphthalide on Autophagy of SH-SY5Y Cells Induced by l-methyl-4-phenyl-pyridiniumion
摘要
Abstract
Objective To observe the effects of butylphthalide on the expression of autophagy-related protein and mRNA in l-meth-yl-4-phenyl-pyridiniumion (MPP+)-induced SH-SY5Y cells, and to explore the protective effect and possible mechanism of butylphthalide to the cell model of Parkinson's disease. Methods The SH-SY5Y cells were divided into control group (A), MPP+group (B), rapamycin pre-treated+MPP+group (C) and Butylphthalide pretreated+MPP+group (D). The relative viability of SH-SY5Y cells induced by MPP+was measured with MTT assay, the morphology of SH-SY5Y cells was observed. The expression of microtubule associated protein 1 light chain 3 (LC3)-II/I and Beclin 1 protein was detected by Western blotting. And the expression of LC3-II/I and Beclin 1 mRNA were assayed by re-al-time quantitative reverse transcription-PCR (RT-PCR). Results The viability rates of cells were significantly lower in group B than in group A (t=20.270, P<0.001), and were significantly higher in groups C and D than in group B (t>8.770, P<0.001), however, there was no significantly difference between groups C and D (t=2.270, P=0.064). The expression of LC3-II/I and Beclin 1 was higher in group B than in group A (t>6.647, P<0.01), and was higher in groups C and D than in group B (t>3.630, P<0.01), however, there was no significantly differ-ence between groups C and D (t<2.238, P≥0.05). Conclusion Butylphthalide could prevent the injury of SH-SY5Y cells induced by MPP+, which may affect Parkinson's disease by inducing autophagy.关键词
丁苯酞/帕金森病/SH-SY5Y细胞/自噬/大鼠Key words
butylphthalide/Parkinson's disease/SH-SY5Y cells/autophagy/rats分类
医药卫生引用本文复制引用
贾玉凤,吴庆文,程月发,陈娟,孟奇..丁苯酞对1-甲基-4-苯基吡啶离子诱导细胞自噬的影响[J].中国康复理论与实践,2016,22(4):422-427,6.基金项目
河北联合大学博士科研启动基金项目(No.35647699)。 （No.35647699）
