中国康复理论与实践2016，Vol.22Issue(4)：428-432,5.DOI:10.3969/j.issn.1006-9771.2016.04.012
脊髓损伤后水通道蛋白-4在脊髓白质中的表达
Expression of Aquaporin-4 in White Matter of Spinal Cord in Rats after Spinal Cord Contusion
摘要
Abstract
Objective To explore the expression of aquaporin (AQP)-4 in white matter of spinal cord after spinal cord contusion (SCC). Methods 88 adult Sprague-Dawley rats were assigned randomly to sham operation group and SCC group. The model was established by Al-len's method. BBB sore was used to assess the motor function of rats. The relative expression of AQP-4 mRNA was determined by Q-PCR technique. The localization of AQP-4 was observed by immunohistochemistry. Results BBB score showed motor dysfunction in SCC group, and it increased 7 and 14 days after SCC (t>5.061, P<0.001). The level of AQP-4 mRNA decreased on the 1st and 3rd days (t>50.44, P<0.001), and increased on the 5th day (t=-3.968, P=0.001), and lasted until the 28th day (t=-4.227, P=0.001) compared with that on the 3rd day. The immunohistochemistry showed AQP-4 was located on the process of glial cell and vascular endothelial cells in white matter of spi-nal cord. Conclusion AQP-4 may play various roles at different stages in SCC.关键词
脊髓挫伤/水肿/水通道蛋白-4/大鼠Key words
spinal cord contusion/edema/aquaporin-4/rats分类
医药卫生引用本文复制引用
向虹雨,李胜男,曾茜,张晓,梁楠..脊髓损伤后水通道蛋白-4在脊髓白质中的表达[J].中国康复理论与实践,2016,22(4):428-432,5.基金项目
四川省级大学生创新创业训练计划项目(No.201313705030)。 （No.201313705030）
