陈犹白 1陈聪慧(综述) 2Qixu Zhang 1韩岩(审校)3
作者信息
- 1. 美国德克萨斯大学MD安德森肿瘤中心整形外科 美国 休斯敦 77030
- 2. 美莱医疗美容医院口腔美容中心 北京 100020
- 3. 解放军总医院整形修复科 中国 北京 100853
- 折叠
摘要
Abstract
Soft tissue defect results from trauma and tumor resection is the common problem in plastic surgery. Existing methods such as tissue flap transplantation, artificial material filling and fat transplantation are not perfect. Adipose-derived stem cells (ASCs) are multipotent stem cells isolated from adipose tissue. Stem cell therapy and adipose tissue engineering based on the adipogenic differentiation capacity of ASCs are promising in plastic and aesthetic surgery and regenerative medicine. The in vitro induction of ASCs differentiation towards adipocyte using medication and chemical was well established. Previous studies showed that various scaffold including decellularized adipose tissue scaffold facilitated ASCs adhesion, proliferation and adipogenic differentiation through mimicking in vivo microenvironment, however its mechanism is not well understood. Factors which could affect ASCs adipocyte differentiation include donor factors such as species, age, gender, anatomic site, and harvesting technique; experimental factors such as cell subpopulation, cell passage, culture condition, and cryopreservation;growth factors such as epidermal growth factor, fibroblast growth factor, vascular endothelial growth factor, insulin-like growth factor, bone morphogenetic proteins, and Nel-like type 1 molecule; hormones such as insulin, glucocorticoids, estrogen, growth hormone, and leptin; chemical like IBMX, paclitaxel and glucagon-like peptide-1;physical factors like radiation and laser; other factors such as Toll-like receptors, platelet-rich plasma and fibrin, human adenovirus-36,and core binding factorα1. Therefore, it is crucial to combine the abovementioned factors and scaffold for improvement of ASCs proliferation and specific differentiation. This review focused on the induction and verification methods, factors and their mechanisms of ASCs adipogenic differentiation. We also introduced our experience and looked forward the future research interest of ASCs adipogenic differentiation based on current issues.关键词
脂肪干细胞/分化/成脂分化/影响因素/机制Key words
Adipose-derived stem cells/differentiation/adipogenic differentiation/factors/mechanism分类
医药卫生
