中国全科医学2016，Vol.19Issue(15)：1792-1795,4.DOI:10.3969/j.issn.1007-9572.2016.15.011
Roux-en-Y胃旁路术对血管内皮细胞P -选择素表达的影响
Effect of Roux-en-Y Gastric Bypass on the Expression of P-selectin in Vascular Endothelial Cells
摘要
Abstract
Objective To observe the influence of Roux - en - Y gastric bypass(RYGB)on the expression of P -selectin by establishing T2DM rat models and to investigate its protection mechanism for vascular endothelial cells. Methods From October 2014 to April 2015,we divided sixty SD rats into three groups:normal control group(NC group),T2DM group and RYGB group,with 20 rats in each group. T2DM group and RYGB group were administrated with high fat diet for three weeks and were given 30 mg/ kg streptozotocin(STZ)by intraperitoneal injection to build T2DM models,and NC group was fed with normal fodder and was given citric acid buffer solution of the same concentration. RYGB was undertaken in RYGB group,and sham operation was undertaken in NC group and T2DM group. The general data of the rats were recorded,including body weight before surgery and 21 days after surgery. The levels of TNF-α and adiponectin concentration were detected by ELISA before surgery and 21 days after surgery;vessel intima thickness was determined by HE staining method 21 days after surgery,and the expression of P - selectin was detected by immunohistochemistry SABC method 21 days after surgery. Results Two rats in RYGB group died from anastomotic obstruction after surgery. T2DM group and RYGB group were lower than NC group in postoperative body weight(P < 0. 05);RYGB group was lower than T2DM group in body weight after surgery(P < 0. 05). NC group had higher body weight after surgery than that before surgery(P < 0. 05);RYGB group had lower body weight after surgery than that before surgery(P < 0. 05). T2DM group was higher in TNF-α level and lower in adiponectin concentration than NC group before and after surgery(P < 0. 05). RYGB group was higher in TNF-α level and lower in adiponectin concentration than NC group before surgery(P < 0. 05);RYGB group was lower in TNF-α level and higher in adiponectin concentration than T2DM group after surgery(P < 0. 05). RYGB group had lower TNF-α level and higher adiponectin concentration after surgery than those before surgery( P < 0. 05) . The three groups were not significantly different in vessel intima thickness( F = 1. 337,P= 0. 271). NC group and RYGB group were lower than T2DM group in the positive rate of P - selectin expression(P < 0. 05). Conclusion RYGB possibly reduces P - selectin expression by adiponectin/ TNF-α signaling pathways,and then reduces the damage of vascular endothelial cells.关键词
糖尿病,2 型/胃旁路术/P 选择素/脂联素Key words
Diabetes mellitus,type 2/Gastric bypass/P-selectin/Adiponectin分类
医药卫生引用本文复制引用
柴芳,齐凤杰,赵树鹏,王月,王俊,李强..Roux-en-Y胃旁路术对血管内皮细胞P -选择素表达的影响[J].中国全科医学,2016,19(15):1792-1795,4.基金项目
辽宁省自然科学基金资助项目（2013022044）；辽宁医学院博士科研启动基金项目（ Y2012B017）；辽宁医学院附属第一医院科技启动基金项目 （）
