中国全科医学2016，Vol.19Issue(15)：1828-1832,5.DOI:10.3969/j.issn.1007-9572.2016.15.018
正天丸对偏头痛模型大鼠三叉神经二级神经元P2X3受体表达的影响研究
Effect of Zhengtian Pills on P2X3 Receptor Expression in Secondary Neurons of Trigeminal Nerve of Rat Model of Migraine
摘要
Abstract
Objective To investigate the effect of Zhengtian pills on P2X3 receptor expression in secondary neurons of trigeminal nerve of rat model of migraine. Methods From September to October in 2014,we selected 30 SPF grade Sprague -Dawle(SD)male rats and using the random number table method divided them into three groups:control group,model group and Zhengtian pills group,with 10 rats in each group. Rats of Zhengtian pills group was given 1. 62 g·kg - 1 ·d - 1 Zhengtian pills by gavage,rats of control group and model group were given 1 m 0. 9% sodium chloride solution by gavage. The drug administration lasted for 7 days. In model group and Zhengtian pills group,migraine rat models were established by subcutaneous injection of nitroglycerin(10 mg/ kg)at the left shoulder at 30 min after the last drug administration. Rats of control group were given 0. 5 ml 0. 9% sodium chloride solution by subcutaneous injection at the left shoulder. Behavioural performances of the rats were observed,including the appearing and disappearing time of red ear and the beginning and ending time of scratching head. Immunoflourescence,Western blotting and Real - time PCR were used to detect the expressions of P2X3 receptor and its mRNA in trigeminocervical complex(TCC). Results Model group and Zhengtian pills group were not significantly different in the appearing time of red ear and the beginning time of scratching head( P > 0. 05 ) . Zhengtian pills group had earlier disappearing time of red ear and earlier ending time of scratching head than model group( P < 0. 05). Immunofluorescence results showed that the expression of P2X3 receptors in TCC was positive in the rats of each group,mainly distributed in the TNC andⅠ,Ⅱ layer of C1 ,C2 cervical spinal dorsal nucleus,and the expression of model group was higher than control group and Zhengtian pills group. The expression levels of P2X3 receptor and its mRNA in TCC of model group and Zhengtian pills group were higher than those of control group(P < 0. 05),while the expression levels of P2X3 receptor and its mRNA of Zhengtian pills group were lower than those of model group(P < 0. 05). Conclusion The effect of Zhengtian pills on the treatment of migraine may be related with its inhibition on the expression of P2X3 receptors in secondary neurons of trigeminal nerve.关键词
偏头痛/三叉神经/神经元/正天丸/P2X3 受体Key words
Migraine disorders/Trigeminal nerve/Neurons/Zhengtian pills/P2X3 receptors分类
医药卫生引用本文复制引用
白方会,李慧,姜婷婷,魏赈权,洪新,陈宝田..正天丸对偏头痛模型大鼠三叉神经二级神经元P2X3受体表达的影响研究[J].中国全科医学,2016,19(15):1828-1832,5.基金项目
国家自然科学基金资助项目（81202687）；广东省科技计划项目（2012B061700018）；广东省中医药管理局科研课题 （）
