中国蔬菜Issue(5):13-17,5.
番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定
Resistance Identification of Tomato Inbred Line‘YNAU335’ to TSWV
摘要
Abstract
Recently,Tomato spotted wilt virus(TSWV)has become a serious disease hazarding tomato production. Identification and selective breeding of disease-resistant varieties is an effective approach to control TSWV. This experiment selected tomato varieties(lines)bred by our group and introduced from other countries,through virus transmission by thrips in greenhouse,and artificial inoculation in illumination incubator for 3 consecutive years to identify the disease resistance against TSWV. The results indicated that inbred line ‘YNAU335’ was immune to TSWV,while the other varieties were all susceptible to TSWV. Furthermore,the nuclear protein gene and small RNA 3′ flank sequences of TSWV in leaves of ‘YNAU335’ were not cloned. On the contrary,these 2 sequences were cloned in other tomato leaves. The results preliminarily demonstrated that ‘YNAU335’ was resistant to TSWV,and could be used as disease resistant tomato resource in resistant breeding against TSWV .关键词
番茄/番茄斑萎病毒/抗病育种/核衣壳蛋白基因/抗性鉴定Key words
Tomato/Tomato spotted wilt virus/Disease resistant breeding/Nucleocapsid protein gene/Resistance identification引用本文复制引用
莫云容,赵凯,张培欣,邓明华,杨正安,朱海山,张宏,汤晓倩,文玲,马仲飞..番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定[J].中国蔬菜,2016,(5):13-17,5.基金项目
国家自然科学基金项目(31460525,31260481),云南省科技计划面上项目(2015FB144),云南省教育厅项目 ()