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番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定

莫云容 赵凯 张培欣 邓明华 杨正安 朱海山 张宏 汤晓倩 文玲 马仲飞

中国蔬菜Issue(5):13-17,5.
中国蔬菜Issue(5):13-17,5.

番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定

Resistance Identification of Tomato Inbred Line‘YNAU335’ to TSWV

莫云容 1赵凯 1张培欣 1邓明华 1杨正安 1朱海山 1张宏 1汤晓倩 1文玲 2马仲飞3

作者信息

  • 1. 云南农业大学园林园艺学院,云南昆明 650201
  • 2. 东川区经济作物技术推广站,云南东川 654100
  • 3. 昭通市昭阳区园艺技术推广所,云南昭通 657000
  • 折叠

摘要

Abstract

Recently,Tomato spotted wilt virus(TSWV)has become a serious disease hazarding tomato production. Identification and selective breeding of disease-resistant varieties is an effective approach to control TSWV. This experiment selected tomato varieties(lines)bred by our group and introduced from other countries,through virus transmission by thrips in greenhouse,and artificial inoculation in illumination incubator for 3 consecutive years to identify the disease resistance against TSWV. The results indicated that inbred line ‘YNAU335’ was immune to TSWV,while the other varieties were all susceptible to TSWV. Furthermore,the nuclear protein gene and small RNA 3′ flank sequences of TSWV in leaves of ‘YNAU335’ were not cloned. On the contrary,these 2 sequences were cloned in other tomato leaves. The results preliminarily demonstrated that ‘YNAU335’ was resistant to TSWV,and could be used as disease resistant tomato resource in resistant breeding against TSWV .

关键词

番茄/番茄斑萎病毒/抗病育种/核衣壳蛋白基因/抗性鉴定

Key words

Tomato/Tomato spotted wilt virus/Disease resistant breeding/Nucleocapsid protein gene/Resistance identification

引用本文复制引用

莫云容,赵凯,张培欣,邓明华,杨正安,朱海山,张宏,汤晓倩,文玲,马仲飞..番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定[J].中国蔬菜,2016,(5):13-17,5.

基金项目

国家自然科学基金项目(31460525,31260481),云南省科技计划面上项目(2015FB144),云南省教育厅项目 ()

中国蔬菜

OA北大核心CSTPCD

1000-6346

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