中国蔬菜Issue(5)：33-37,5.
茎瘤芥（榨菜）根肿病不同发病时期根际土壤真菌群落变化特征的高通量分析
High Throughput Analysis on Change Characteristics of Rhizosphere Soil Fungal Community during Different Occurance Times of Tuber Mustard Clubroot Disease
摘要
Abstract
Tuber mustard clubroot is a soil-borne disease and closely related with soil fungal community. In this study,the change regulation of soil fungal community during different occurance times of tuber mustard clubroot(pre-transplanting,premorbid after transplant,early and middle stages of disease occurance,after harvest)was sequenced by 454 high-throughout method using 18S rDNA sequence of soil fungi as target.The results showed that average of 1 056 OTUs(Operational Taxonomic Units)were obtained from one soil sample. According to taxonomy of each OTU,the dominant group wasAscomycetes,followed byBasidiomycota.The diversity indexes of Shannon and Simpson,numbers of OTU and genera in premorbid after transplanting,early and middle stages were all higher than that in pre-transplanting stage and after harvesting stage.The results indicated that the diversity of soil fungal community is higher in the period of tuber mustard clubroot development. It might be related to the vigorous production of root exudate of tuber mustard.关键词
茎瘤芥/根肿病/土壤真菌/高通量测序/多样性指数Key words
Tumorous stem mustard/Clubroot/Soil fungi/High-throughout sequencing/Diversity index引用本文复制引用
王殿东,谭永忠,田雪亮,张超,秦明一,潘丽梅..茎瘤芥（榨菜）根肿病不同发病时期根际土壤真菌群落变化特征的高通量分析[J].中国蔬菜,2016,(5):33-37,5.基金项目
国家自然科学基金项目（31170463），重庆市青年骨干教师自助计划项目 （）
