中国比较医学杂志2016，Vol.26Issue(2)：27-32,6.DOI:10.3969/j.issn.1671-7856.2016.02.006
生物安全实验室病毒悬液灭活试验方法标准转化研究
Transformation and standardization of the inactivation test method for virus suspension in biosafety laboratory
摘要
Abstract
Chemical disinfectants are generally used for virus inactivation and environment disinfection in biosafety laboratory, and the efficacy and evaluation of the disinfection are critical to ensure the laboratory biosafety.However, there is a current lack of applied standard to evaluate the virucidal efficacy of chemical disinfectants in our country.In this paper, a European Union standard“Method and Requirements of Virucidal Quantitative Suspension Test Method for Chemical Disinfectants Used in Human Medicine” was analyzed and a standard transformation scheme has been proposed.It is suggested that the model viruses should be increased from 3 to 6, including the surrogate viruses to substitute highly pathogenic viruses, and that the method to remove the residual chemical disinfectant and the calculation of 95%confidence interval should be incorporated into the standard.The suggestion will improve the scientific and operational standards related to disinfection and sterilization in biosafety laboratory in China.关键词
病毒灭活/化学消毒剂/生物安全/标准/定量悬浮试验/杀病毒效果评估Key words
Virus inactivation/Chemical disinfectant/Biosafety/Standard/Quantitative suspension test/Virucidal effect evaluation分类
医药卫生引用本文复制引用
邓阿妹,魏凤,吴佳,马海霞,袁志明..生物安全实验室病毒悬液灭活试验方法标准转化研究[J].中国比较医学杂志,2016,26(2):27-32,6.基金项目
“十二五”国家卫生行业专项项目（2012ZX10004403）；国家自然科学基金项目（71103178）；中国科学院优秀人才择优支持项目；武汉市科技项目“武汉市新发传染病与生物安全重点实验室”（201261638501）。 （）
