中国动物传染病学报2016，Vol.24Issue(2)：1-7,7.
猪繁殖与呼吸综合征病毒JX14T2毒株的分离与鉴定
CHARACTERIZATION OF A NEW HIGHLY PATHOGENIC JX14T2 STRAIN OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS
摘要
Abstract
To investigate the etiological agent causing high fever, dyspnea and death of fattening pigs on a pig farm in Jiangxi province, a total of 20 blood and tissue samples were collected from dead pigs and examined in RT-PCR. As a result, 19 out of 20 samples were positive for Porcine reproductive and respiratory syndrome virus (PRRSV). Then, one lung sample that was positive in RT-PCR was inoculated to Marc-145. The typical PRRSV-induced cytopathic effect (CPE) developed after 48 hours of inoculation. The viruses at the fifth passage were demonstrated to be PRRSV in RT-PCR and IFA. Therefore, the isolate was confirmed to be PRRSV designate as strain JX14T2. In addition, the strain JX14T2 showed similar characters to the highly pathogenic strains in formation of CPE and plague and growth curve in Marc-145. The sequence analysis of the strain JX14T2 showed that its complete genome was 14960 bp and belonged to North American genotype. A continuous deletion of 120 aa between residues 598-717 was identified in NSP2 protein, in which the 1+29 aa deletion existed that was typically found in HP-PRRSV. The characterization of the strain JX14T2 added new information for further understanding of epidemiology of PRRSV.关键词
猪繁殖与呼吸综合征病毒/JX14T2/分离/鉴定Key words
Porcine reproductive and respiratory syndrome virus/JX14T2/isolation/identification分类
农业科技引用本文复制引用
王鑫,杨德强,张文超,姜一峰,虞凌雪,杨莘,高飞,李丽薇,黄勤峰..猪繁殖与呼吸综合征病毒JX14T2毒株的分离与鉴定[J].中国动物传染病学报,2016,24(2):1-7,7.基金项目
973计划(2014CB542702) （2014CB542702）
国家科技支撑计划课题(2015BAD12B01-1) （2015BAD12B01-1）
国家生猪现代产业技术体系项目(CARS-36) （CARS-36）
国家自然科学基金(31302098、31300140、31502072) （31302098、31300140、31502072）
上海市闵行区人才发展专项资金 （）
