中国动物传染病学报2016，Vol.24Issue(2)：8-12,5.
伪狂犬病毒的microRNAs靶基因预测及功能鉴定
TARGET PREDICTION AND FUNCTIONAL IDENTIFICATION OF PSEUDORABIES VIRUS-ENCODED MICRORNAS
摘要
Abstract
In order to understand the function of microRNA (miRNA) encoded by Pseudorabies virus (PRV), the objective of the present study was to define potential vmiRNA-gene interaction based on bioinformatic analysis in combination with the dual luciferase reporter assay system. The results showed prv-miR-LLT1, prv-miR-LLT7 and prv-miR-LLT9 targeted the transcription activator IE180 gene and prv-miR-LLT7 targeted the UL48 gene involved in viral DNA replication. These vmiRNAs might have regulatory functions in the course of PRV infection.The results were of significance for further research on functions of vmiRNAs regarding virus replication, immune evasion and latent infection.关键词
伪狂犬病毒/微小RNA/靶基因Key words
Pseudorabies virus/microRNA/target gene分类
农业科技引用本文复制引用
刘飞,童武,郑浩,李国新,梁超,郑旭晨,田青,童光志..伪狂犬病毒的microRNAs靶基因预测及功能鉴定[J].中国动物传染病学报,2016,24(2):8-12,5.基金项目
上海市自然科学基金项目(14ZR1448900) （14ZR1448900）
上海市科技兴农重点攻关项目(沪农科攻字(2015)第1-7号) （沪农科攻字(2015）
中央级公益性科研院所基本科研业务费专项资金项目(2014JB02) （2014JB02）
