中国动物传染病学报2016，Vol.24Issue(2)：47-52,6.
日本血吸虫钙网质蛋白活化宿主巨噬细胞的初步研究
PRELIMINARY STUDY ON EFFECT OFSCHISTOSOMA JAPONICUM CALRETICULIN PROTEIN ON ACTIVATION OF MACROPHAGOCYTES
摘要
Abstract
The objective of the present study was to investigate into the functions that Schistosoma japonicum calreticulin protein (SjCRT) activated mouse macrophagocytes. Insect cells Sf9 were used to express SjCRT. Macrophagocytes were stimulated with SjCRT for 72 h. The expression levels of macrophagocyte surface chemokine receptor CCR7 were assayed with flow cytometry. The expression levels of COX-2, cPLA2, TNF-α and IL-1β mRNAs were assayed in RT-PCR. The ELISA was used to assay the concentration of TNF-α and Griess to assay the concentration of NO in the surpernatant samples. The flow cytometry results show that SjCRT significantly promoted up-regulation of the CCR7 expression (P<0.05) in macrophagocytes. RT-PCR results indicated that SjCRT upregulated mRNA levels of COX-2, cPLA2, TNF-α and IL-1β. According to the results of ELISA and Griess,SjCRT significantly stimulated macrophagocytes to secret NO. In brief,SjCRT activated mouse macrophagocytes that might be involved in the response to schistosomiasis vaccination.关键词
日本血吸虫/钙网质蛋白/巨噬细胞/趋化因子受体/细胞因子Key words
Schistosoma japonicum/calreticulin protein/macrophagocyte/chemochines recepters/cytokine分类
农业科技引用本文复制引用
马丽贞,李丹丹,苑纯秀,艾敏,史晓娜,塔娜,冯新港..日本血吸虫钙网质蛋白活化宿主巨噬细胞的初步研究[J].中国动物传染病学报,2016,24(2):47-52,6.基金项目
中国农业科学院创新团队基金 （）
