中国医科大学学报2016，Vol.45Issue(4)：289-292,297,5.DOI:10.12007/j.issn.0258-4646.2016.04.001
Wnt/β-catenin信号通路中Wnt基因对肾癌细胞的生物学影响
Biological Effects of Wnt in Wnt/β-catenin Signaling Pathway on Kidney Cancer Cell
摘要
Abstract
Objective To investigate the biological effects of Wnt gene in kidney cancer Caki⁃2 cells. Methods The Wnt gene was silenced in kidney cancer Caki⁃2 cells by lentivirus vector. The cell proliferate ability of cells in each group were assayed by CCK⁃8 kit at different time points. The apoptosis of Caki⁃2 cells was observed after silencing Wnt gene by transmission electron microscope. The invasion ability of each group cells was tested using Transwell chambers. The genes expression changes of Wnt/β⁃catenin signaling pathway and apoptosis related gene were determined by realtime PCR. Results Compared with the other two groups,the cell proliferate ability of the cells after silencing Wntgene was suppressed,and the difference was statistically significant(P<0.05). Apoptosis increased significantly in shRNA+Caki⁃2+Wnt group cells with silencing of Wntgene, and apoptotic body appeared in these cells. In invasive experiment,the number of emigrated cells in shRNA+Caki⁃2+Wnt group were significantly lower than other groups(P<0.05). The expression of Wnt mRNA,β⁃catenin mRNA and Bcl⁃2 mRNA in shRNA+Caki⁃2+Wnt group cells was lower than other groups(P<0.05). Conclusion Silencing of Wnt gene of kidney cancer Caki⁃2 cells can affect the proliferation rate of the cells, promote the cell apoptosis,and inhibit the invasion ability,which provide certain theoretical basis for the research and development of new drugs and new therapeutic targets.关键词
肾癌/Wnt/β-catenin/Bcl-2Key words
kidney cancer/Wnt/β-catenin/Bcl-2分类
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孟凡东,李妍,吴迪,蒋涛,王扬,隋承光,姜又红..Wnt/β-catenin信号通路中Wnt基因对肾癌细胞的生物学影响[J].中国医科大学学报,2016,45(4):289-292,297,5.基金项目
国家自然科学基金（81202955）；辽宁省教育厅高校重点实验室支持计划项目 （）
