中国医科大学学报2016，Vol.45Issue(4)：293-297,5.DOI:10.12007/j.issn.0258-4646.2016.04.002
人乳头瘤病毒16型甲基化水平与子宫颈病变程度关系的研究
Correlation between Severity of Cervical Lesions and Methylation of Human Papilloma Virus Type 16 DNA
摘要
Abstract
Objective To quantifiably measure the methylation frequency of 18 CpG sites in the 3′region of L1 gene and long control region(LCR) gene of HPVl6 DNA,and study the relationship between HPVl6 DNA methylation and severity of cervical lesions. Methods A total of 10 cases Normal/low⁃grade squamous intraepithelial lesion(Normal/LSIL),10 cases of high⁃grade squamous intraepithelial lesion(HSIL),and 10 cases of cervical cancer(CC)were recruited for the study. The relationship between severity of cervical lesions and HPV16 DNA methylation was analyzed by bisultlte⁃pyrosequencing. Results The methylation rate was highest in Normal/LSIL at position 7 089 located in 3′⁃L1,followed by CC. The low⁃est was found in HSIL. The difference in methylation percentage among the three lesions was significant(P=0.006). In 7 134,the proportion meth⁃ylation was also different among three groups(P=0.01),difference in methylation percentage between Normal/LSIL and CC,as well as Normal/LSIL and HSIL was significant(P=0.038,0.017). Conclusion The methylation status of CpG sites 7 089 and 7 134 in the 3′region of L1gene is asso⁃ciated with the severity of cervical disease. The quantification of HPV DNA methylation can be used for cervical disease screening in clinical samples.关键词
人乳头瘤病毒16/甲基化/CpG位点/子宫颈病变/焦磷酸测序Key words
human papilloma virus16/methylation/CpG site/cervical lesion/pyrosequencing分类
医药卫生引用本文复制引用
王微,刘建华,王桂丽,齐特,阮强,孙峥嵘..人乳头瘤病毒16型甲基化水平与子宫颈病变程度关系的研究[J].中国医科大学学报,2016,45(4):293-297,5.基金项目
国家自然科学基金 （）
