中国医科大学学报2016，Vol.45Issue(4)：324-327,4.DOI:10.12007/j.issn.0258-4646.2016.04.009
PP242对人晶状体上皮细胞凋亡相关蛋白Bcl-2表达的影响
Effects of PP242 on the Expression of Apoptosis Protein Bcl-2 in Human Lens Epithelial Cells
摘要
Abstract
Objective To explore the effects of PP242 on the expression of apoptosis protein Bcl⁃2 in human lens epithelial cells(HLECs). Meth⁃ods Immortal HLECs SRA01/04 were cultured and treated with different concentrations of PP242. The cell growth was examined by MTT assay at 24 h and 48 h after PP242 treatment. Real⁃time quantitative polymerase chain reaction(RT⁃PCR)and Western blot were adopted to detect the mRNA and protein expression of Bcl⁃2 respectively. Results The cultured HLECs SRA01/04 were collected and treated with different concentra⁃tions(100,250,500,750,1 000,1 500 nmol/L)of PP242. After treatment for 24 h and 48 h,the inhibitory rate in each well was determined by MTT assay. The inhibition rate was as below,24 h:7.55%,9.43%,16.98%,22.64%,26.42%,30.19%;48 h:11.11%,23.81%,36.51%,42.86%, 49.21%,63.49%. Compared with the control group,the difference was statistically significant(P<0.05). Compared with the control group(0 nmol/L),the expression of Bcl⁃2 protein was significantly decreased with the increasing concentrations of PP242(P<0.05),while the expression of Bcl⁃2 mRNA was inhibited by PP242 based on the results of RT⁃PCR. Compared with the control group(0 nmol/L),the RT⁃PCR results of Bcl⁃2 mRNA were 0.723±0.039,0.517±0.028,0.353±0.052,0.167±0.046,respectively(P<0.05). Conclusion PP242 could inhibit cell proliferation of HLECs in vitro with a concentration and time depended manner. Bcl⁃2 protein is expressed in HLECs,which could be down regulated by PP242 treatment.关键词
PP242/人晶状体上皮细胞/Bcl-2Key words
PP242/human lens epithelial cells/Bcl-2分类
医药卫生引用本文复制引用
白雪,冯浩,张柳,杨志博,宁宏..PP242对人晶状体上皮细胞凋亡相关蛋白Bcl-2表达的影响[J].中国医科大学学报,2016,45(4):324-327,4.基金项目
辽宁省社会发展攻关计划 （）
