中国医科大学学报2016，Vol.45Issue(5)：402-405,4.DOI:10.12007/j.issn.0258-4646.2016.05.005
原癌基因MET启动子的G-四链体结构
G-quadruplex Structures in Promoters of MET Proto-oncogene
摘要
Abstract
Objective To identify G⁃quadruplex structures in the promoter region of MET. Methods CD spectroscopy,UV spectroscopy,non⁃denatured electrophoresis and PCR stop assay were applied to indicate the G⁃quadruplex structure and its function. Results The Pu23WT se⁃quence in the promoter of MET adopted an intramolecular parallel G⁃quadruplex structure under physiological conditions in vitro,which can stop the extension of Pmet. Conclusion G⁃quadruplex structure in the promoter might inhibit MET gene expression in vivo.关键词
G-四链体/原癌基因MET/基因表达调控Key words
G-quadruplex/MET proto-oncogene/regulation of gene expression分类
医药卫生引用本文复制引用
阎敬,关一夫..原癌基因MET启动子的G-四链体结构[J].中国医科大学学报,2016,45(5):402-405,4.基金项目
国家自然科学基金 （）
