中国药理学通报2016，Vol.32Issue(5)：620-624,625,6.DOI:10.3969/j.issn.1001-1978.2016.05.007
CUS致大鼠抑郁行为涉及TNF-α及IDO-HAAO通路激活
TNF-αmediated activation of IDO pathway involved in mechanism of depression in rats induced by CUS
摘要
Abstract
Aim To investigate the relationship be-tween the depression-like behaviors of rats induced by chronically unpredictable stress( CUS) and the protein expression of tumor necrosis factor alpha( TNF-α) , in-dole-2 , 3-dioxygenase ( IDO ) and 3-hydroxyl amino acid oxygenase( HAAO) . Methods Thirty male Spra-gue Dawley( SD) rats were randomly divided into con-trol group and model group. CUS plus solitary condi-tion were used to establish the depression model. The open field test ( OFT ) and the force swimming test ( FST ) were used to evaluate the depression-like behaviors of rats ;Western blot was used to determine the protein expressions of TNF-α, IDO and HAAO in the prefrontal cortex and hippocampus of rats. Results Model group rats showed obvious depression-like be-haviors with increasing immobile time in FST ( P <0. 01) and decreasing locomotive activity in OFT( P<0. 01 ) , and up-regulating the protein expression of TNF-α, IDO and HAAO in the prefrontal cortex and hippocampus significantly ( P <0. 05 ) , compared with control group rats. Conclusion The depression-like behaviors of rats induced by CUS may be associated with the activation of IDO-HAAO pathway mediated by TNF-α.关键词
抑郁症/慢性不可预见刺激/肿瘤坏死因子α/吲哚胺2 ,3-双加氧酶/3-羟氨苯甲酸双加氧酶/细胞因子Key words
depression/CUS/TNF-α/IDO/HAAO/cytokines分类
医药卫生引用本文复制引用
伏箫燕,李海燕,崔婷,蒋青松,邱红梅..CUS致大鼠抑郁行为涉及TNF-α及IDO-HAAO通路激活[J].中国药理学通报,2016,32(5):620-624,625,6.基金项目
国家自然科学基金资助项目( No 31400881) （ No 31400881）
重庆市教委科学技术项目(No KJ1400208) （No KJ1400208）
