中国药理学通报2016，Vol.32Issue(5)：625-630,631,7.DOI:10.3969/j.issn.1001-1978.2016.05.008
表皮生长因子受体在肾血管性高血压大鼠心血管重构中的作用
Epidermal growth factor receptor involvement in cardiovascular remodeling in renovascular hypertensive rats
摘要
Abstract
Aim To determine the possibilities and mechanisms of EGFR, a receptor protein tyrosine kinase associated with many important cellular processes re-sponsible for cardiovascular remodeling in renovascular hypertensive rats. Methods 2K1C hypertensive rats were used in the present study. Blood pressure was measured with the tail-cuff method. LVMI and his-topathological changes in the cardiovascular system were analysed. EGFR expressions of aorta and myocar-dium as well as phosphorylation levels of ERK in hy-pertensive rats were detected by immunohistochemistry and Western blot analysis, respectively. Results Sys-tolic blood pressure was markedly increased 2 weeks after 2K1C surgery. Cardiovascular remodeling induced by hypertension was confirmed by elevated LVMI, pro-liferation of collagen fibers in myocardial interstitium, histopathological changes in cardiovascular system and increased IMT of thoracic aorta 6 weeks after 2 K1 C surgery. Compared with sham rats, EGFR expression in the ventricular myocardium of 2 K1 C rats was signifi-cantly increased at 6 weeks ( P<0. 05 ) , and the EG-FR/GAPDH ratio was higher in 2 K1 C rats with higher systolic blood pressure ( P < 0. 05 ) . Phosphorylation level of ERK 1/2 was upregulated correspondingly in 2 K1 C rats ( P <0. 01 ) . Increased EGFR expression was also found in aortas of 2K1C rats, particularly in tunica intima and media. Conclusion EGFR and its down-stream kinases ERK 1/2 are involved in cardio-vascular remodeling in association with the severity of hypertension in renovascular hypertensive rats.关键词
两肾一夹(2K1C)/肾血管性高血压/心血管重构/表皮生长因子受体/MAPK/大鼠Key words
two-kidney, one-clip (2K1C)/renovas-cular hypertension/cardiovascular remodeling/epider-mal growth factor receptor/mitogen-activated protein kinases/rat分类
医药卫生引用本文复制引用
冯梅,滕文浩,姜雯雯,向继洲,刘慧,杨磊..表皮生长因子受体在肾血管性高血压大鼠心血管重构中的作用[J].中国药理学通报,2016,32(5):625-630,631,7.基金项目
国家自然科学基金资助项目( No 81370299) （ No 81370299）
中央高校基础研究基金(No 2012QN135) （No 2012QN135）
广州医科大学博士启动基金(No 2012C04) （No 2012C04）
