中国药理学通报2016，Vol.32Issue(5)：664-669,670,7.DOI:10.3969/j.issn.1001-1978.2016.05.014
左卡尼汀抑制NF-κB敏化TRAIL诱导神经胶质瘤细胞凋亡
L-carnitine sensitizes human glioblastoma cells to TRAIL-induced apoptosis
摘要
Abstract
Aim To investigate the enhancing effect of L-carnitine as a sensitizer on tumor necrosis factor-re-lated apoptosis inducing ligand ( TRAIL)-induced ap-optosis in glioma cells. Methods Glioma cell U87 was used as model cell line. Cell viability was determined by CCK-8 , and apoptosis was assessed by Annexin V-FITC/PI staining, caspase-3 activity and expres-sion. The expression and transcription of nuclear factor kappa B ( NF-κB ) and FLICE inhibiting protein ( c-FLIP) were measured by RT-PCR and Western blot. In addition, NF-κB was knockdown to analyze its regu-lating effect on c-FLIP expression. Results The com-bination treatment with TRAIL and L-carnitine signifi-cantly inhibited cell proliferation and induced apopto-sis. Compared with control, combinational treatment significantly suppressed the transcription and expres-sion of c-FLIP as well as translocation of NF-κB. Through silencing NF-κB, NF-κB was found to act as upstream signaling to regulate c-FLIP. Conclusion L-carnitine sensitizes TRAIL-induced tumor cell apoptosis via suppression of NF-κB-dependent c-FLIP expres-sion.关键词
左卡尼汀/TRAIL/NF-κB/c-FLIP/神经胶质瘤/凋亡Key words
L-carnitine/TRAIL/NF-κB/c-FLIP/GBM/apoptosis分类
医药卫生引用本文复制引用
杨秀伟,谢靖,钟凤,韩彦弢..左卡尼汀抑制NF-κB敏化TRAIL诱导神经胶质瘤细胞凋亡[J].中国药理学通报,2016,32(5):664-669,670,7.基金项目
国家自然科学基金资助项目(No 81473384) （No 81473384）
