中国药理学通报2016，Vol.32Issue(5)：681-686,687,7.DOI:10.3969/j.issn.1001-1978.2016.05.017
瞬时感受器电位离子通道香草素受体亚家族4调控肝星状细胞活化增殖作用研究
Regulatory effects of TRPV4 on liver fibrosis of rats
摘要
Abstract
Aim To investigate the effect of TRPV4 on hepatic fibrosis of rats . Methods Liver fibrosis model of rats was induced by 50% CCl4 twice a week for 12 weeks. HE and Masson staining were used to evaluate the degree of hepatic fibrosis, and the levels of α-smooth muscle actin(α-SMA) and TRPV4 were detec-ted in fibrotic liver tissue by Western blot. HSC-T6 cells were activated by transforming growth factor β1 (TGF-β1),and the protein levels of α-SMA, TRPV4 were detected by Western blot. After using Ru and transfected with TRPV4-siRNA, HSC-T6 was stimula-ted with TGF-β1, the levels of α-SMA, TRPV4 and phosphorylation level of Akt were determined by West-ern blot. Results TRPV4 was highly expressed in model liver tissues and in activated HSC-T6 induced by TGF-β1 . The levels of α-SMA and phosphorylation of Akt decreased in TGF-β1-induced HSC, used with Ru or transfected with TRPV4-siRNA. Conclusions The expression of TRPV4 increases in fibrotic livers and ac-tivated hepatic stellate cells. Knockdown of TRPV4 can suppress the activation of hepatic stellate cells in-duced by TGF-β1 , and decrease the phosphorylation levels of Akt.关键词
肝纤维化/肝星状细胞/TRPV4/Akt/钌红/活化增殖Key words
liver fibrosis/hepatic stellate cell/TR-PV4/Akt/Ru/activation and proliferation分类
医药卫生引用本文复制引用
宋阳,詹磊,黄成,李俊..瞬时感受器电位离子通道香草素受体亚家族4调控肝星状细胞活化增殖作用研究[J].中国药理学通报,2016,32(5):681-686,687,7.基金项目
国家自然科学基金资助项目(No 81473268,81273526) （No 81473268,81273526）
教育部博士点基金项目( No 20123420120001) （ No 20123420120001）
安徽省自然科学基金项目(No 1408085MKL31) （No 1408085MKL31）
安徽省科技攻关项目(No 1301042212) （No 1301042212）
