中国药理学通报2016，Vol.32Issue(5)：697-702,6.DOI:10.3969/j.issn.1001-1978.2016.05.020
抑郁大鼠海马组织的比较蛋白质组学研究
Analysis of CUMS-induced differentially expressed proteins in rat hippocampus by ITRAQ
摘要
Abstract
Aims To compare hippocampus between CUMS rats and normal rats, to find differentially ex-pressed proteins and to explore the pathogenesis of de-pression in the protein levels and biological marker. Methods Chronic unpredicted mild stress was taken to establish rat depression model. The ITRAQ-labeled proteins and peptides were separated by the cation col-umn, and differentially expressed proteins were detec-ted and identified by 2D LC-MS/MS. The functions of proteins were analyzed by bioinformatics. Results To-tally 5 109 proteins were identified, 33 differentially expressed proteins were identified, the expressions of 8 proteins were increased and 25 proteins downregulated. Conclusion ITRAQ based sereening is effective in discovering the nosogenesis of depression and new bio-logical marker.关键词
ITRAQ/蛋白质组学/抑郁/海马组织/质谱/CUMSKey words
ITRAQ/proteomics/depression/hippo-campus/mass spectrum/CUMS分类
医药卫生引用本文复制引用
曹莉莎,张芳,罗文,陈静,何淼泉,王继生..抑郁大鼠海马组织的比较蛋白质组学研究[J].中国药理学通报,2016,32(5):697-702,6.基金项目
四川省卫生厅科研课题(No 120320) （No 120320）
