中华耳科学杂志2016，Vol.14Issue(1)：15-20,6.DOI:10.3969/j.issn.1672-2922.2016.01.004
荣昌猪电子耳蜗植入方法建立及听功能初步观察
Establish the standard method of Rongchang pig cochlear implant and preliminary observation of hearing function
摘要
Abstract
Objective Establish the standard method of cochlear implant on large mammals–pigs, and observed the changes of ABR&EABR before and after cochlear implants. Methods Six Rongchang pigs, male and female un-limited, 40-45 days, 8~12 kg, were selected from Key Laboratory of Pig Industry Sciences (Ministry of Agriculture), Chongqing Academy of Animal Science. Divided into the normal hearing group (Mitf+/+), and the mutation deafness group (Mitf-/-), each group of three. Cochlear implantation were operated in general anesthesia. ABR&EABR were recorded prior to surgery, immediately finish surgery and 1 week after. Observed the position of electrodes by Skull X-ray. Results Cochlear implant is succeed in six animals, electrode position is good, within the cochlea coiling 1.5~1.75 turns. Cochlear implants immediately, operation side (right), waveform of ABR can’t be recorded. Seven days af-ter the operation, operation side (right), ABR threshold is 100 dB SPL at low frequency, but waveform of ABR can’t be recorded at high frequency still. Cochlear implants immediately and a week later, EABR threshold is 90 CL at normal hearing group (Mitf+/+). It was lower than 190 CL of group of mutation deafness(Mitf-/-). Conclusion Our study show that the methods of cochlear implant at Rongchang pig is really practical, and the EABR test via electrodes im-planted is convenient and feasible. Our study provide the basis for more directly research on cochlear implants in vivo and the physiological or pathological changes of cochlear after cochlear implanted.关键词
耳蜗植入/荣昌猪/ABR/EABRKey words
Cochlear implant/Rongchang pig/ABR/EABR分类
医药卫生引用本文复制引用
陈伟,陈磊,杨仕明,刘日渊,张亮,冀飞,郭维维,任丽丽,李佳楠,袁硕龙,张悦..荣昌猪电子耳蜗植入方法建立及听功能初步观察[J].中华耳科学杂志,2016,14(1):15-20,6.基金项目
国家973计划重大科学研究计划干细胞项目（2012CB967900）；国家973计划重大科学问题导向项目（2011CBA01000）；国家自然科学基金面上项目（81271082）；国家自然科学基金青年项目（81400472）；北京科技创新基地培育与发展专项；重庆市基本科研业务费项目（11611，14440）；空军总医院课题（KZ2015005）。 （）
