中华耳科学杂志2016，Vol.14Issue(1)：32-36,5.DOI:10.3969/j.issn.1672-2922.2016.01.007
老年性聋耳蜗带状突触数量在时空上的改变
Time and frequency dependent changes of cochlear ribbon synapses in age-related hearing loss
王驰 1柳柯 2赵立东 2候琨 2施磊 3杨仕明 2宁博1
作者信息
- 1. 江苏省徐州市中心医院耳鼻咽喉科 江苏221009
- 2. 解放军总医院耳鼻咽喉头颈外科，解放军耳鼻咽喉研究所 北京100853
- 3. 中国医科大学附属第一医院耳鼻咽喉头颈外科 沈阳110001
- 折叠
摘要
Abstract
Objective To study possible correlations between the number of cochlear ribbon synapses and elevation of ABR thresholds related to aging. Methods C57BL/6J mice were utilized to mimic age related hearing loss in this study. ABR threshold was examined at 1M, 2M, 4M, 6M, and 12M respectively. Immunostaining combined with laser confocal mi-croscopy was used to identify RIBEYE/CtBP2, a specific pre-synaptic component of cochlear ribbon synapses, and the num-ber of labeling synaptic ribbons was calculated using the 3D max 8.0 software. Results ABR threshold elevation was first found after 4M (P<0.05), and continued to increase at 6M and 12M (P<0.05). Further, the most significant ABR threshold el-evation at a given age was identified at high frequencies (P<0.01, 8 and 16 kHz). Correspondingly, the reduction of labeling ribbon synapses was noticed along with ABR threshold shifts as the mice aged, also with more labeling synapses reduction in the high frequency regions (P<0.01, 8 and 16 kHz). However, we did not find significant loss of inner hair cells in during the same aging period. Conclusion There is faithful correlation between hearing threshold shifts and reduction of labeling synaptic ribbons, with most reduction occurring in high frequency regions. This study shows that the loss of ribbon synapses may contribute to age-related hearing loss.关键词
老年性聋/C57小鼠/耳蜗带状突触/RIBEYE/CtBP2/内毛细胞Key words
Age-related hearing loss/C57 mice/Cochlear ribbon synapse/RIBEYE/CtBP2/Inner hair cell分类
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王驰,柳柯,赵立东,候琨,施磊,杨仕明,宁博..老年性聋耳蜗带状突触数量在时空上的改变[J].中华耳科学杂志,2016,14(1):32-36,5.
