中华临床免疫和变态反应杂志2016，Vol.10Issue(1)：46-51,6.DOI:10.3969/j.issn.1673-8705.2016.01.009
组织蛋白酶S在原发性干燥综合征中的临床意义
Clinical Significance of Cathepsin S in Primary Sjögren’s Syndrome
摘要
Abstract
Objective To explore the clinical significance of cathepsin (Cat)S in primary Sjögren’s syndrome (pSS ). Methods Indirect enzyme-linked immunosorbent assay was used to detect the serum concentrations of total-and pro-Cat S respectively in 37 pSS patients and 33 healthy controls.The relation between the serum levels of Cat S and EULAR Sjögren’s syndrome disease activity index (ESSDAI)and the serum levels of immunoglobulin (Ig)G were analyzed.Immunohistochemical analysis of labial gland tissue was performed to detect the expression of Cat S.The data were analyzed by student’s t-test,spearman correlation analysis,and receiver operating characteristic (ROC)curve was used to obtain an optimal cutoff value.Results The serum levels of total-Cat S,pro-Cat S and active-Cat S in pSS group(n =37)were significantly higher than those in healthy controls(n =33)[total-Cat S:(18.70 ±2.53)ng/ml vs.(15.10 ±3.16)ng/ml;pro-Cat S:(14.14 ±3.56)ng/ml vs.(12.20 ±2.92)ng/ml;active-Cat S:(4.56 ±2.49) ng/ml vs.(2.90 ±1.95)ng/ml,P <0.05].Moreover,no difference was observed in the serum levels of total-Cat S,pro-Cat S and active-Cat S between the subgroups of pSS patients based on the IgG levels (P >0.05).In addition,the serum levels of total-Cat S,pro-Cat S and active-Cat S in pSS group were not correlated with the levels of IgG and ESSDAI (P >0.05).The area under ROC curve (AUC)of total-Cat S,pro-Cat S and active-CatS was 0.811 (P <0.01 ),0.655 (P <0.01 ),and 0.701 (P <0.01 ), respectively.The optimal cut-off value was 15.77 ng/ml,12.62 ng/ml and 4.062 ng/ml,respectively, with the sensitivity of 0.919,0.676, and 0.622 and specificity of 0.697, 0.667, and 0.758. Immunohistochemical analysis of labial gland tissue of pSS patients showed positive expression of Cat S which was distributed in the infiltrated lymphocytes,acinar cells and ductual epithelial cells while not in controls.Conclusion Cat S may play an important role in the pathogenesis of pSS by enhancing the autoantigen presentation,degradation of extracellular matrix and infiltration of lymphocytes.It may be a new serological marker and potential therapeutic target.关键词
原发性干燥综合征/组织蛋白酶S/生物标志物/唇腺活检Key words
primary Sjögren’s syndrome/cathepsin S/biomarker/lip biopsy分类
医药卫生引用本文复制引用
李霞,张丽丽,费允云,赵岩..组织蛋白酶S在原发性干燥综合征中的临床意义[J].中华临床免疫和变态反应杂志,2016,10(1):46-51,6.基金项目
国家自然科学基金（81471614），协和青年基金（PUMC Youth Fund）和中央高校基本科研业务费专项资金 （）
