组织工程与重建外科杂志2016，Vol.12Issue(2)：94-97,4.DOI:10.3969/j.issn.1673-0364.2016.02.002
犬骨髓单个核细胞构建组织工程骨的体外实验研究
Experimental Study of Constructing Tissue Engineered Bone with Canine Bone Marrow Mononuclear Cells in Vitro
尹宏宇 1丁榆德 1刘广鹏 2曹谊林3
作者信息
- 1. 100144 北京市 中国医学科学院整形外科医院颌面整形外科中心
- 2. 200072 上海市 上海市第十人民医院整形外科
- 3. 100144 北京市 中国医学科学院整形外科医院
- 折叠
摘要
Abstract
Objective To explore a method for instantly constructed TEB to meet the instantly clinical requirements of repairing bone defects. Methods BMNCs of Beagle were separated by 1.063 g/mL Percoll gradient solution. The ideal seeding density of 30 ×106 cells/mL was obtained for canine bone marrow mononuclear cells (cBMNCs) on partially demineralized bone matrix (pDBM). The cell activity and osteogenic capacity of immediately constructed TEB composed of cBMNCs and pDBM were analyzed in vitro. Results Certain cell activity and osteogenic capacity were confirmed in immediately constructed TEB under the osteogenically inducted environment in vitro. Conclusion The directly replantation of instantly constructed TEB might satisfy the immediately clinical requirements of repairing bone defects.关键词
组织工程骨/骨髓单个核细胞/骨髓基质干细胞Key words
Tissue engineered bone/Bone marrow mononuclear cells/Bone marrow mesenchymal stem cells分类
生物科学引用本文复制引用
尹宏宇,丁榆德,刘广鹏,曹谊林..犬骨髓单个核细胞构建组织工程骨的体外实验研究[J].组织工程与重建外科杂志,2016,12(2):94-97,4.
