波谱学杂志2016，Vol.33Issue(2)：224-235,12.DOI:10.11938/cjmr20160205
不同病程的结核病患者的血浆代谢组学研究
Plasma Metabonomics in Tuberculosis Patients at Different Stages
摘要
Abstract
Tuberculosis (TB) is the second largest infectious disease caused by a single pathogen leading to mortality, only after AIDS. Discovery of biomarkers associated with disease progression is vital for TB screening and control. Metabonomics is among the most useful tools for this purpose. A few metabonomic studies on TB patients have been published. In this study, the plasma samples of TB patients with varying severity were studied by1H NMR based metabonomics. Orthogonal partial least squares-discriminant analysis (OPLS-DA) models showed that TB patients at different stages and healthy controls could be differentiated by their plasma metabolic profiles. It was found that the TB groups had significantly higher levels of valine, alanine, creatine, and 3-hydroxybutyrate, relative to the healthy controls. In the TB patients, the levels of lactate, pyruvate, N-acetyl glycoprotein, leucine and glutamate increased gradually with disease progression, while the level of citrate, a tricarboxylic acid (TCA) cycle intermediate, decreased with disease progression. The plasma metabolic profile changes indicated increased energy metabolism and glycolysis, enhanced ketogenesis of fatty acid, reduced TCA cycle, and disordered amino acid metabolism in the TB patients.关键词
核磁共振(NMR)/代谢组学/正交偏最小二乘法判别分析(OPLS-DA)/结核病/差异代谢物Key words
NMR/metabonomics/OPLS-DA/tuberculosis/differential metabolites分类
数理科学引用本文复制引用
周宁,杜祥博,杨莉,许怡,孙博,张琪,戴二黑,颜贤忠,董方霆..不同病程的结核病患者的血浆代谢组学研究[J].波谱学杂志,2016,33(2):224-235,12.基金项目
国家科技重大专项资助项目(2013ZX10003002-006) （2013ZX10003002-006）
