南方医科大学学报2016，Vol.36Issue(6)：735-743,9.DOI:10.3969/j.issn.1673-4254.2016.06.01
siRNA特异性沉默ADP核糖基化因子6对前列腺癌PC-3细胞增殖、迁移和侵袭的影响
Small RNA interference-mediated ADP-ribosylation factor 6 silencing inhibits proliferation, migration and invasion of human prostate cancer PC-3 cells
摘要
Abstract
Objective To investigate the effects of silencing ADP-ribosylation factor 6 (Arf6) on the proliferation, migration, and invasion of prostate cancer cell line PC-3 and the possible molecular mechanisms. Methods Three Arf6-specific small interfering RNA (siRNA) were transfected into cultured prostate cancer cell line PC-3. Arf6 expression was examined by real-time PCR and Western blotting. MTT assay, wound healing assay, and Transwell migration and invasion assay were used to observe the effect of Arf6 silencing on the proliferation, migration, and invasion ability of PC-3 cells. The levels of phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), ERK1/2, p-AKT, AKT and Rac1 were detected by Western blotting. Results Transfection of siRNA-3 resulted in significantly decreased Arf6 mRNA and protein expression with inhibition rates of (91.88 ± 3.13)% and (86.37 ± 0.57)%, respectively. Arf6 silencing by siRNA-3 markedly suppressed the proliferation, migration and invasion of PC-3 cells and reduced the expression levels of p-ERK1/2 and Rac1. Conclusion Silencing of Arf6 efficiently inhibits the proliferation, migration, and invasion of PC-3 cells in vitro, and the underlying mechanisms may involve the down-regulation of p-ERK1/2 and Rac1.关键词
ADP核糖基化因子6/前列腺癌/侵袭/迁移/RNA干扰Key words
ADP-ribosylation factor 6/prostate cancer/invasion/migration/RNA interference引用本文复制引用
单雄威,吕世栋,于小明,胡正飞,张嘉杰,王广发,魏强..siRNA特异性沉默ADP核糖基化因子6对前列腺癌PC-3细胞增殖、迁移和侵袭的影响[J].南方医科大学学报,2016,36(6):735-743,9.基金项目
Supported by Natural Science Foundation of Guangdong Prov-ince (S2013010014537) and by Science and Technology Planning Project of Guangdong Province (2012B031800263,2014A010107012,2014A020212260).基金项目广东省自然科学基金（S2013010014537）；广东省科技计划 （S2013010014537）
