南方医科大学学报2016，Vol.36Issue(6)：751-755,5.DOI:10.3969/j.issn.1673-4254.2016.06.03
p16INK4a蛋白可作为乳腺癌特异性分子标志
p16INK4a protein is a specific molecular biomarker of breast cancer
摘要
Abstract
Objective To investigate the expression of p16INK4a protein in breast cancer and analyze its clinical significance. Methods A total of 132 surgical specimens of primary breast cancer obtained between 2014 and 2015 were examined for expressions of ER, PR, CK5/6, Her-2 and p16INK4a proteins using immunohistochemistry. Results The breast cancer samples were classified into 5 molecular subtypes, namely Luminal A (58 cases), Luminal B (32 cases), Her-2-positive (21 cases), basal-like (12 cases) and normal-like (9 cases) types. p16INK4a expression was negative in 7/132 (5.30%) cases, weakly positive in 15/132 (11.36%) cases, positive in 40/132 (30.30%) cases, and strongly positive in 70/132 (53.03%) cases. When categorizing negative and weakly positive cases into negative group and the positive and strongly positive cases into positive group, the total negative and positive expression rates of p16INK4a were 16.67%(22/132) and 83.33%(110/132) in the carcinoma tissues. Statistical analysis showed the expression intensity of p16INK4a differed significantly between the age groups (P<0.05) but was not significantly correlated with ER, PR, Her-2, molecular subtypes or metastasis of the tumors. Conclusion The compensatory high expression of p16INK4a is the main mechanism of cell cycle deregulation in invasive breast cancer and can be an important specific molecular marker for invasive breast cancer.关键词
乳腺癌/p16INK4a/细胞周期/免疫组化/分子标志Key words
breast cancer/p16INK4a/cell cycle/immunohistochemistry/molecular target引用本文复制引用
杨军,黄小钟,郭睿,黄莺,康安静,靳耀锋,陈晓黎,李宗芳..p16INK4a蛋白可作为乳腺癌特异性分子标志[J].南方医科大学学报,2016,36(6):751-755,5.基金项目
国家自然科学基金（NSFC30872403）；教育部新世纪优秀人才计划（NCET-10-0647）；教育部“长江学者和创新团队发展计划”创新团队（IRT1171） Supported by National Natural Science Foundation of China (NSFC30872403) （NSFC30872403）
