南方医科大学学报2016，Vol.36Issue(6)：756-762,7.DOI:10.3969/j.issn.1673-4254.2016.06.04
5-羟色胺-7受体激动剂对帕金森病模型大鼠内侧前额叶皮层锥体神经元兴奋性的影响
Effect of 5-HT7 receptor agonist on pyramidal neurons in the medial frontal cortex in a rat model of Parkinson's disease
摘要
Abstract
Objective To investigate the activity of pyramidal neurons in the medial prefrontal cortex (mPFC) of normal and 6-OHDA-lesioned rats and the responses of the neurons to 5-hydroxytryptamine-7 (5-HT7) receptor stimulation. Methods The changes in spontaneous firing of the pyramidal neurons in the mPFC in response to 5-HT7 receptor stimulation were observed by extracellular recording in normal and 6-OHDA-lesioned rats. Results Both systemic and local administration of 5-HT7 receptor agonist AS 19 resulted in 3 response patterns (excitation, inhibition and no change) of the pyramidal neurons in the mPFC of normal and 6-OHDA-lesioned rats. In normal rats, the predominant response of the pyramidal neurons to AS 19 stimulation was excitatory, and the inhibitory effect of systemically administered AS 19 was reversed by GABAA receptor antagonist picrotoxinin. In the lesioned rats, systemic administration of AS 19 also increased the mean firing rate of the pyramidal neurons, but the cumulative dose for producing excitation was higher than that in normal rats. Systemic administration of AS 19 produced an inhibitory effect in the lesioned rats, which was partially reversed by picrotoxinin. Local administration of AS 19 at the same dose did not change the fi ring rate of the neurons in the lesioned rats. Conclusion The activity of mPFC pyramidal neurons is directly or indirectly regulated by 5-HT7 receptor, and degeneration of the nigrostriatal pathway leads to decreased response of these neurons to AS 19.关键词
帕金森病/内侧前额叶皮层/锥体神经元/5-羟色胺-7受体/大鼠Key words
Parkinson's disease/medial prefrontal cortex/pyramidal neuron/5-hydroxytryptamine-7 receptor引用本文复制引用
范玲玲,邓博,闫君宝,胡志红,任爱红,胡咏梅,杨东伟..5-羟色胺-7受体激动剂对帕金森病模型大鼠内侧前额叶皮层锥体神经元兴奋性的影响[J].南方医科大学学报,2016,36(6):756-762,7.基金项目
河南省教育厅科学技术研究重点项目（14B180007）；洛阳市科技发展计划项目 （）
