南方医科大学学报2016，Vol.36Issue(6)：763-767,5.DOI:10.3969/j.issn.1673-4254.2016.06.05
虎杖苷对ApoE-/-小鼠肝脏miR-214表达水平及肝功能的影响
Effect of polydatin on miR-214 expression and liver function in ApoE-/-mice
摘要
Abstract
Objective To study the effect of polydatin on the expression level of miR-214 and liver function in atherosclerotic mice. Methods Forty male ApoE-/- mice were randomly allocated into 4 groups (n=10), namely the model group, low- and high-dose polydatin groups, and simvastin group, with 10 male C57BL/6J mice serving as the normal control group. Mouse models of atherosclerosis were established by feeding the ApoE-/-mice with a high-fat diet. After 12 weeks of treatment, blood levels of glucose, lipids, AST, and ALT and the contents of T-SOD and MDA in the liver tissue were detected. The pathologies of the liver were examined with HE staining, and miR-214 expression in the liver was detected using quantitative real-time PCR. Results Compared with the normal control mice, the mice in the model group showed significantly increased blood glucose, serum TC, TG, LDL-C, ALT, and AST levels, and MDA contents in the liver (P<0.01), with significantly decreased serum HDL-C level and SOD and miR-214 levels in liver (P<0.01). Polydatin treatment significantly ameliorated such changes in blood glucose, serum ALT, AST, TC, TG, LDL-C, and HDL-C levels, and MDA, SOD, and miR-214 contents in liver tissue (P<0.05). Conclusions Polydatin can reduce blood glucose and lipid levels and protect the liver function in atherosclerotic mice possibly by up-regulating the expression of miR-214 and T-SOD and down-regulating MDA in the liver.关键词
虎杖苷/动脉粥样硬化/miR-214/肝功能/氧化应激Key words
polydatin/atherosclerosis/miR-214/liver function/oxidative stress引用本文复制引用
周凤华,温子云,何泽淮,李梅,尹琼丽,石成钢,成彩联..虎杖苷对ApoE-/-小鼠肝脏miR-214表达水平及肝功能的影响[J].南方医科大学学报,2016,36(6):763-767,5.基金项目
国家自然科学基金青年基金（81403339）；广东省自然科学基金博士启动项目（2014A030310150）；广东省中医药管理局科研项目（20141186） Supported by Natural Science Foundation for the Youth (NSFY) of China (81403339) （NSFY）
