南方医科大学学报2016，Vol.36Issue(6)：790-795,6.DOI:10.3969/j.issn.1673-4254.2016.06.10
NLRP3炎症小体介导血管紧张素Ⅱ诱导的人脐静脉血管内皮细胞炎症因子IL-1β的表达
NLRP3 inflammasome mediates angiotension II-induced expression of inflammatory factor interleukin-1βin human umbilical vein endothelial cells
摘要
Abstract
Objective To investigate the effect of angiotension II (AngII) on the activation of NLRP3 inflammasome and the expression of interleukin-1β(IL-1β) in human umbilical vein endothelial cells (HUVECs). Methods HUVECs cultured in vitro were treated with different concentrations of AngII for varying lengths of time to determine the optimal concentration and time for AngII exposure. To test the impact of different agents on the effect of AngII exposure, HUVECs were pretreated with AngII receptor blocker losartan, NAD(P)H inhibitor DPI and H2O2 scavenger CAT, caspase 1 inhibitor YVAD, or NLRP3 siRNA for silencing NLRP3, and the protein levels of NOX4, NLRP3, caspase-1 and IL-1β in HUVECs were analyzed by Western blotting. Results AngII treatment at the optimal concentration (10-9 mol/L) for 12 h significantly increased the protein levels of NOX4, NLRP3, caspase1 and IL-1βin HUVECs. Pretreatment with losartan, DPI, CAT, YVAD, or NLRP3 siRNA all attenuated the effects of AngII on the cells. Conclusion AngII can induce vascular inflammation by promoting the production of reactive oxygen species and activating NLRP3 inflammasome to increase the protein expression of IL-1βin HUVECs.关键词
血管紧张素Ⅱ/脐静脉内皮细胞/活性氧/NLRP3炎症小体/白介素-1βKey words
angiotension II/human umbilical vein endothelial cells/reactive oxygen species/NLRP3 inflammasome/interleukin-1β引用本文复制引用
杨仁强,黄玲,马晓欣,金思一,王丹,李旭..NLRP3炎症小体介导血管紧张素Ⅱ诱导的人脐静脉血管内皮细胞炎症因子IL-1β的表达[J].南方医科大学学报,2016,36(6):790-795,6.基金项目
国家自然科学基金（30770974） Supported by National Natural Science Foundation of China (30770974) （30770974）
