南方医科大学学报2016，Vol.36Issue(6)：802-806,5.DOI:10.3969/j.issn.1673-4254.2016.06.12
siRNA干扰相互作用蛋白1表达抑制胶质瘤细胞的侵袭和迁移
RNA interference of PC4 and SFRS1 interacting protein 1 inhibits invasion and migration of U87 glioma cells
摘要
Abstract
Objective To investigate the effect of small interfering RNA (siRNA)-mediated silencing of PC4 and SFRS1 interacting protein 1 (PSIP1) on invasion and migration of human glioma U87 cells. Methods Chemically synthesized siRNA targeting PSIP1 gene was transfected into U87 cells via lipofectamine, and the gene silencing effect was determined using real-time PCR. The changes in the invasion and migration abilities of the transfected cells were assessed with Transwell assay and wound healing assay, respectively. Western blotting was used to analyze the expression of N-cadherin,β-catenin and the transcription factor Slug. Results The mRNA and protein level of PSIP1 was significantly reduced in U87 cells after transfection with PSIP1 siRNA (P<0.0001). PSIP1 knockdown in U87 cells resulted in significant suppression of cell invasion and migration abilities (P<0.01) and also reduced N-cadherin,β-catenin and Slug expressions. Conclusions Silencing of PSIP1 impairs the invasion and migration abilities of glioma cells and lowers the expressions of N-cadherin, β-catenin and Slug, suggesting that PSIP1 may regulate Slug by classical Wnt/β-catenin signaling pathway to modulate epithelial-mesenchymal transition and promote the invasion and migration of glioma cells.关键词
U87/siRNA干扰/相互作用蛋白1/侵袭/间质转化Key words
U87/RNA interference/PC4 and SFRS1 interacting protein 1/epithelial-mesenchymal transition/invasion引用本文复制引用
向伟,漆松涛,刘亚伟,李和珍,周强,易国仲,陈子阳,严乐..siRNA干扰相互作用蛋白1表达抑制胶质瘤细胞的侵袭和迁移[J].南方医科大学学报,2016,36(6):802-806,5.基金项目
国家自然科学基金（81372692，81472315，81572498），广东省自然科学基金（2014A030313167） Supported by National Natural Science Foundation of China (81372692,81472315,81572498) （81372692,81472315,81572498）
