南方医科大学学报2016，Vol.36Issue(6)：848-856,9.DOI:10.3969/j.issn.1673-4254.2016.06.21
DHA对宫颈癌细胞株凋亡、侵袭和转移的影响
Effects of docosahexaenoic acid on cell apoptosis, invasion and migration of cervical cancer cells in vitro
摘要
Abstract
Objective To investigate the effect of docosahexaenoic acid (DHA) on apoptosis, migration and invasion of cervical cancer cell lines. Methods cervical cancer cell lines Hela and Siha in logarithmic phase were treated different concentrations of DHA. The morphological changes of the cells were observed microscopically and cell apoptosis was observed using Hoechst 33258 fluorescent staining. MTT assay was used to evaluate the effect of DHA in suppressing cell growth, and flow cytometry was employed to analyze the changes of cell apoptotic rate following DHA stimulations. Wound healing assay and Transwell migration assay were used to evaluate the migration of the cell lines. The expression levels of Bax, Bcl-2 cleaved caspase3, MMP-9 and VEGF proteins were detected by Western blotting. Results DHA exposure of the cells caused obvious morphological changes and dose-dependently increased the number of apoptotic bodies in the cells. MTT assay showed that DHA inhibited the growth of the cancer cells in a time-and concentration-dependent manner. DHA also effectively suppressed migration and invasion of the cancer cells. The cells exposed to DHA showed significantly down-regulation of Bcl-2, MMP-9 and VEGF proteins and up-regulation of cleaved-caspase 3 and Bax. Conclusion DHA can promote cervical carcinoma cell apoptosis by down-regulating the anti-apoptotic proteins Bax, Bcl-2 and cleaved-caspase3 and suppress cell invasion by decreasing MMP-9 and VEGF expressions.关键词
宫颈肿瘤/DHA/细胞凋亡/细胞侵袭/细胞转移Key words
cervical carcinoma/docosahexaenoic acid/apoptosis/invasion/migration引用本文复制引用
杨翠,张广平,陈咏宁,孟凡良,刘士三,龚时鹏..DHA对宫颈癌细胞株凋亡、侵袭和转移的影响[J].南方医科大学学报,2016,36(6):848-856,9.基金项目
广东省科技计划项目（2016A020215115）；花都区科技计划产学研协同创新重点专项 （）
