南方医科大学学报2016，Vol.36Issue(6)：870-874,5.DOI:10.3969/j.issn.1673-4254.2016.06.24
HBV转基因小鼠体内淋巴细胞表型特点及干扰素α对其的影响
Characteristics of lymphocyte phenotypes in HBV transgenic mice and the effect of interferon-α:a preliminary study
摘要
Abstract
Objective To analyze the characteristics of lymphocyte phenotypes in hepatitis B virus (HBV) transgenic mice and the effect of exogenous interferon-αon virological profiles and lymphocytes phenotypes of the mice. Methods HBV transgenic mice and wild-type (WT) mice were examined for serum levels of HBsAg, HBcAb, IL-21, and IL-6 using ELISA. The frequencies of CD4+T and CD19+B cells separated from the liver, spleen, and peripheral blood were detected by flow cytometry. Nine HBV transgenic mice were injected subcutaneously with recombinant mouse interferon alpha (rmIFN-α) and another 9 transgenic mice were injected with PBS, and their HBsAg, HBV DNA, IL-6, and IL-21 levels and frequencies of peripheral blood CD4+T and CD19+B cells were detected. Results HBV transgenic mice showed a high level of HBsAg with a detectable level of HBcAb and significantly increased serum levels of IL-21 and IL-6 as compared with WT mice (P<0.05). The transgenic mice had a significantly lower frequency of CD4+T cells in the peripheral blood, liver and spleen (P<0.05) but a significantly higher frequency of CD19+B cells in the liver (P<0.05). An inverse correlation between intrahepatic CD4+T cell frequency and serum HBsAg level while a positive correlation between intrahepatic CD19+B cell frequency and HBcAb level were found in HBV transgenic mice. Administration of rmIFN-α significantly increased the frequencies of CD4+ T and CD19+ B cells in the peripheral blood and the serum level of IL-6 in HBV transgenic mice (P<0.05). Conclusion HBV transgenic mice have lymphocyte subset dysregulation and exogenous interferon-αcan modulate the immune function of the mice by regulating the frequencies of lymphocyte subsets.关键词
乙型肝炎病毒/转基因小鼠/干扰素α/免疫调节Key words
hepatitis B virus/transgenic mouse/interferon alpha/immunoregulation引用本文复制引用
严鑫,钟锐华,刘锦红,周扬,唐利波,李咏茵,刘光泽,侯金林..HBV转基因小鼠体内淋巴细胞表型特点及干扰素α对其的影响[J].南方医科大学学报,2016,36(6):870-874,5.基金项目
广东省自然科学基金 （）
